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PMID: 6258164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A cDNA clone from Zea mays endosperm sucrose synthetase mRNA.

Nucleic acids research ·Vol. 8 ·No. 24 ·1980-12-20 ·Pages 6175-88

Geiser M, Döring HP, Wöstemeyer J, Behrens U, Tillmann E, Starlinger P

Abstract

A cDNA clone for maize endosperm sucrose synthetase of 62o nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endosperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoter translation. In Southern blotting to genomic BamHI-digested DNA, a single band only of approximately 20 Kb lights up, indicating that the sucrose synthetase gene is unique, or that closely linked copies are located on this DNA fragment.

MeSH Terms
Base Composition Cloning, Molecular DNA/isolation & purification,metabolism DNA Restriction Enzymes Escherichia coli/genetics Fructose/genetics Glucosyltransferases/genetics Nucleic Acid Hybridization Plants/genetics Plasmids Protein Biosynthesis RNA, Messenger/metabolism Zea mays
Chemicals
RNA, Messenger Fructose DNA Glucosyltransferases sucrose synthase DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Geiser M
Döring H P
Wöstemeyer J
Behrens U
Tillmann E
Starlinger P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.24.6175
Published
1980-12-20
Pages
6175-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328080
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.24.6175 PMC Full Text View journal details
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