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PMID: 6287423 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of maize genes coding for zein proteins of the 21000 dalton size class.

Nucleic acids research ·Vol. 10 ·No. 13 ·1982-07-10 ·Pages 3845-60

Pintor-Toro JA, Langridge P, Feix G

Abstract

Cloning in lambda gt WES phage of EcoRI fragments from maize seedlings DNA led to the isolation of four fragments containing genes coding for 21000 dalton zein proteins. The zein genes, identified by electron microscopic analysis, do not contain intervening sequences detectable by this method. The flanking sequences were analyzed by restriction sites mapping and hybridization and showed areas of homology between each other and with sequences surrounding a previously isolated gene of the 19000 dalton zein class.

MeSH Terms

Cloning, Molecular DNA/genetics,isolation & purification DNA Restriction Enzymes Genes Microscopy, Electron Molecular Weight Nucleic Acid Conformation Nucleic Acid Hybridization Plants/genetics Zea mays/genetics Zein/genetics

Chemicals

DNA Zein DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pintor-Toro J A

Langridge P

Feix G

References (13)

13 references, click to expand

Complex organization of zein genes in maize.
Gene. 1981 Apr;13(3):239-49 PMID: 7262557
Electrophoretic fractionation and translation in vitro of poly(rA)-containing RNA from maize endosperm. Evidence of two mRNAs coding for zein protein.
Eur J Biochem. 1978 Dec;92(2):605-11 PMID: 738282
Cloned genomic segments of Zea mays homologous to zein mRNAs.
Gene. 1981 Aug;14(3):205-15 PMID: 6269962
Multiplicity and diversity of cloned zein cDNA sequences and their chromosomal localisation.
EMBO J. 1982;1(1):53-8 PMID: 16453407
Restriction cleavage map of SP01 DNA: general location of early, middle, and late genes.
J Virol. 1979 Jul;31(1):156-71 PMID: 116010
Genes and mRNAs coding for zein polypeptides in Zea mays.
Eur J Biochem. 1979 Dec;102(1):211-22 PMID: 520323
EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.
Science. 1977 Apr 8;196(4286):175-7 PMID: 322278
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Zein synthesis in maize endosperm by polyribosomes attached to protein bodies.
Proc Natl Acad Sci U S A. 1976 Feb;73(2):515-9 PMID: 1061153
The primary structure of a plant storage protein: zein.
Nucleic Acids Res. 1981 Oct 10;9(19):5163-74 PMID: 6895552
Purification and translation of zein messenger RNA from maize endosperm protein bodies.
Proc Natl Acad Sci U S A. 1978 Feb;75(2):696-700 PMID: 16592496
Cloning of double stranded DNAs derived from polysomal mRNA of maize endosperm: isolation and characterisation of zein clones.
Nucleic Acids Res. 1979 Jun 25;6(8):2707-15 PMID: 461201
Zein storage protein gene family of maize. An assessment of heterogeneity with cloned messenger RNA sequences.
J Mol Biol. 1982 Jan 5;154(1):33-49 PMID: 6896217

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.13.3845

Published

1982-07-10

Pages

3845-60

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320763

Subset

External Links

PubMed source DOI: 10.1093/nar/10.13.3845 PMC Full Text View journal details

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Isolation and characterization of maize genes coding for zein proteins of the 21000 dalton size class.

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