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PMID: 6190127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The characterization of cDNA clones coding for wheat storage proteins.

Nucleic acids research ·Vol. 11 ·No. 10 ·1983-05-25 ·Pages 2961-77

Bartels D, Thompson RD

Abstract

Poly(A)+ RNA isolated from the developing wheat endosperm var. Chinese Spring, has been used as template for the construction of a cDNA library. Within the library, clones have been identified by in vitro translation of hybrid-selected mRNA which encode alpha/beta gliadin related sequences and gamma-gliadin related sequences. The DNA sequence of one such clone has been determined and it shows homology with that of a clone encoding a barley storage protein, B-hordein. The sequence includes a tandem DNA repeat which is discussed in relation to the generation of diversity within the gliadins.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/analysis Molecular Weight Nucleic Acid Hybridization Plant Proteins/genetics Plants/genetics Plasmids Poly A/genetics Protein Biosynthesis RNA/genetics RNA, Messenger Triticum/genetics
Chemicals
Plant Proteins RNA, Messenger storage protein, Triticum aestivum Poly A RNA DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bartels D
Thompson R D
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20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.10.2961
Published
1983-05-25
Pages
2961-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325941
Subset
IM
Databases
GENBANK
J01309
External Links
PubMed source DOI: 10.1093/nar/11.10.2961 PMC Full Text View journal details
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