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PMID: 118435 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Filter replicas and permanent collections of recombinant DNA plasmids.

Nucleic acids research ·Vol. 7 ·No. 8 ·1979-12-20 ·Pages 2115-36

Gergen JP, Stern RH, Wensink PC

Abstract

A permanent, ordered collection of 23,000 recombinant DNA plasmids containing Drosophila melanogaster DNA has been established. Simple and practical methods for storing and manipulating this collection were developed. In addition, an improved, simple and inexpensive method for making paper filter replicas of such an ordered collection and of a high density (10,000 colonies/petri dish) unordered collection was developed. These filter replicas are suitable for nucleic acid hybridization screens of recombinant DNA colinies and each filter replica can be used for many (greater than 5) successive screens. The kinetics of this hybridization reaction were examined and allow design of experiments that detect colony complementarity to a nucleic acid that is 0.5% of the hybridization probe.

MeSH Terms
Animals Cloning, Molecular DNA, Recombinant Drosophila melanogaster Kinetics Methods Nucleic Acid Hybridization Paper Plasmids
Chemicals
DNA, Recombinant
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gergen J P
Stern R H
Wensink P C
References (36)
36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.8.2115
Published
1979-12-20
Pages
2115-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342374
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.8.2115 PMC Full Text View journal details
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