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PMID: 2823102 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes.

Molecular and cellular biology ·Vol. 7 ·No. 8 ·1987-08-00 ·Pages 2708-17

Friden P, Schimmel P

Abstract

Although the majority of genes for amino acid biosynthesis which have been examined are under general amino acid control, LEU1 and LEU2 of Saccharomyces cerevisiae respond specifically to leucine. We report here an analysis of LEU3, a putative leucine-specific regulatory locus. We show that LEU3 is necessary for expression of wild-type levels of LEU1- and LEU2-specific RNAs and, further, that the levels of LEU4-specific transcripts are also affected by LEU3. We cloned LEU3 and showed by DNA sequence analysis that it contained an open reading frame of 886 amino acids. A striking feature of the predicted LEU3 protein was a cluster of acidic amino acids (19 of 20) located in the C-terminal half of the coding region. The protein also had a repeated cysteine motif which was conserved in a number of other yeast proteins implicated in gene regulation. We show that whole-cell extracts contained a LEU3-dependent DNA-binding activity that interacted with the 5' region of LEU2. Subdivision of the LEU2 5' region established that the LEU3-dependent DNA-binding activity interacted with the segment which had the previously reported homology with LEU1.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Deletion DNA Restriction Enzymes DNA-Binding Proteins Fungal Proteins Gene Expression Regulation Genes, Fungal Leucine/biosynthesis Nucleic Acid Hybridization Plasmids RNA, Fungal/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Trans-Activators
Chemicals
DNA-Binding Proteins Fungal Proteins LEU3 protein, S cerevisiae RNA, Fungal Saccharomyces cerevisiae Proteins Trans-Activators DNA Restriction Enzymes Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Friden P
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Schimmel P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.8.2708-2717.1987
Published
1987-08-00
Pages
2708-17
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367887
Subset
IM
Grants
NIGMS NIH HHS · GM-15539 · United States
Databases
GENBANK
M17222
External Links
PubMed source DOI: 10.1128/mcb.7.8.2708-2717.1987 PMC Full Text View journal details
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