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PMID: 3027658 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The ILV5 gene of Saccharomyces cerevisiae is highly expressed.

Nucleic acids research ·Vol. 14 ·No. 24 ·1986-12-22 ·Pages 9631-51

Petersen JG, Holmberg S

Abstract

The nucleotide sequence of the yeast ILV5 gene, which codes for the branched-chain amino acid biosynthesis enzyme acetohydroxyacid reductoisomerase, has been determined. The ILV5 coding region is 1,185 nucleotides, corresponding to a polypeptide with a molecular weight of 44,280. Transcription of the ILV5 mRNA initiates at position -81 upstream from the ATG translation start codon and terminates between 218 and 222 bases downstream from the stop codon. Consensus sequences have been identified for initiation and termination of transcription, and for general control of amino acid biosynthesis, as well as repression by leucine. The ILV5 gene is regulated slightly by general amino acid control. Codon usage of the ILV5 gene has the strong bias observed in yeast genes that are highly expressed. In agreement with this, the reductoisomerase monomer, with an apparent molecular weight of 40,000, has been identified in an SDS polyacrylamide gel pattern of total soluble yeast proteins as a gene dosage dependent band.

MeSH Terms
2-Acetolactate Mutase/genetics Amino Acid Sequence Base Sequence DNA Restriction Enzymes Genes Genes, Fungal Isomerases/genetics Nucleotide Mapping Plasmids Saccharomyces cerevisiae/enzymology,genetics Transcription, Genetic
Chemicals
DNA Restriction Enzymes Isomerases 2-Acetolactate Mutase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Petersen J G
Holmberg S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.24.9631
Published
1986-12-22
Pages
9631-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341325
Subset
IM
Databases
GENBANK
X04969
External Links
PubMed source DOI: 10.1093/nar/14.24.9631 PMC Full Text View journal details
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