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PMID: 4562389 Published · ppublish English Journal Article

Isoleucine and valine metabolism in Escherichia coli. 18. Induction of acetohydroxy acid isomeroreductase.

Journal of bacteriology ·Vol. 112 ·No. 1 ·1972-10-00 ·Pages 131-41

Ratzkin B, Arfin S, Umbarger HE

Abstract

The regulation by substrate induction of the acetohydroxy acid isomeroreductase was studied in Escherichia coli. Induction was inhibited by chloramphenicol and rifampin. The addition of rifampin resulted in a decay of the capacity to form isomeroreductase. This was attributed to the breakdown of the isomeroreductase messenger, which had a half-life of about 45 sec at 37 C. Induction of isomeroreductase was enhanced by including glucose in the medium. This effect was shown to be due in part to the lowering of the pH of the medium, which presumably made inducer entry more rapid.

MeSH Terms
Cell-Free System Chloramphenicol/pharmacology Culture Media Enzyme Induction/drug effects Enzyme Repression/drug effects Escherichia coli/enzymology,growth & development,metabolism Glucose/metabolism Glycerol/metabolism Hydro-Lyases/metabolism Hydrogen-Ion Concentration Hydroxybutyrates/pharmacology Isoleucine/biosynthesis Isomerases/biosynthesis,metabolism Lactates/pharmacology Lyases/metabolism Microbiology/instrumentation Mutation Rifampin/pharmacology Threonine Valine/biosynthesis
Chemicals
Culture Media Hydroxybutyrates Lactates Isoleucine Threonine Chloramphenicol Lyases Hydro-Lyases Isomerases Valine Glucose Glycerol Rifampin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ratzkin B
Arfin S
Umbarger H E
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.112.1.131-141.1972
Published
1972-10-00
Pages
131-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251388
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.112.1.131-141.1972 PMC Full Text View journal details
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