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PMID: 6275358 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The actin gene in yeast Saccharomyces cerevisiae: 5' and 3' end mapping, flanking and putative regulatory sequences.

Nucleic acids research ·Vol. 9 ·No. 23 ·1981-12-11 ·Pages 6339-50

Gallwitz D, Perrin F, Seidel R

Abstract

The 5' and 3' flanking regions of the yeast actin gene have been sequenced and the ends of the actin mRNA were determined by the single-strand nuclease mapping procedure. The mRNA starts with a pyrimidine residue 141 (or 140) nucleotides upstream from the initiation codon. The actin gene lacks a typical "TATA" box 30 base pairs upstream from the mRNA start site but it contains a region homologous to the canonical sequence 5'-GGCTCAATCT-3' which is found in several eukaryotic genes 70 to 80 bp upstream from the mRNA cap site. Judging from the S1 nuclease mapping, there are two populations of actin mRNA terminating 98 and 107 nucleotides downstream from the stop codon. The 3' termini are preceded by three AATAAA sequences found in most eukaryotic polyadenylated mRNAs.

MeSH Terms
Actins/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Microscopy, Electron RNA, Messenger/genetics Saccharomyces cerevisiae/genetics
Chemicals
Actins DNA, Recombinant RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gallwitz D
Perrin F
Seidel R
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.23.6339
Published
1981-12-11
Pages
6339-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327607
Subset
IM
Databases
GENBANK
J01310, L00026
External Links
PubMed source DOI: 10.1093/nar/9.23.6339 PMC Full Text View journal details
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