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PMID: 2987874 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

(TAA)n within sequences flanking several intrachromosomal variant surface glycoprotein genes in Trypanosoma brucei.

Nucleic acids research ·Vol. 13 ·No. 9 ·1985-05-10 ·Pages 3161-77

Aline R, MacDonald G, Brown E, Allison J, Myler P, Rothwell V, Stuart K

Abstract

In Trypanosoma brucei telomeric copies of intrachromosomal variant surface glycoprotein (VSG) genes are produced through a gene conversion mechanism and are expressed if the telomere is or becomes transcriptionally activated. We have analyzed a sequence that occurs 1 to 1.5 kb 5' to several intrachromosomal VSG genes. This flanking sequence has three distinct sections: a 5' section containing 5 to 116 TAA or TAA-like tandem repeats; a moderately conserved sequence with an alternating GT characteristic; and a highly conserved 3' sequence that is largely alternating TA. Restriction mapping data and the location of this sequence suggest that it occurs at or near the 5' gene conversion boundary. This sequence, however, is not 5' to all VSG genes. These data suggest that this flanking sequence alone is not sufficient for VSG gene expression but that it may function in gene conversion for many, but not all, VSG genes.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes/metabolism Gene Conversion Gene Expression Regulation Glycoproteins/genetics Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Trypanosoma brucei brucei/genetics Variant Surface Glycoproteins, Trypanosoma
Chemicals
Glycoproteins Variant Surface Glycoproteins, Trypanosoma DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Aline R
MacDonald G
Brown E
Allison J
Myler P
Rothwell V
Stuart K
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.9.3161
Published
1985-05-10
Pages
3161-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341227
Subset
IM
Grants
NIAID NIH HHS · AI 17375 · United States
Databases
GENBANK
X02426, X02427, X02428
External Links
PubMed source DOI: 10.1093/nar/13.9.3161 PMC Full Text View journal details
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