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PMID: 6083548 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The IsTat 1.3 VSG multigene family in Trypanosoma brucei: retention of the expression linked copy through multiple antigenic switches.

Nucleic acids research ·Vol. 12 ·No. 23 ·1984-12-11 ·Pages 9051-66

Allison J, Rothwell V, Newport G, Agabian N, Stuart K

Abstract

In the IsTaR 1 serodeme of T. brucei the 3 variant surface glycoprotein (VSG) gene family contains about 10 members, one of which has a telomeric location on a minichromosome. The expression linked copy (ELC) of the 3 VSG gene which occurs in an antigenic variant expressing the 3 VSG, also has a telomeric location but unlike the minichromosomal 3 VSG gene has restriction sites upstream from the 5' barren region. This ELC is retained on the same telomere in a subsequent variant that expresses a telomeric 7 VSG ELC and in relapse variants and procyclic forms derived from variant antigenic types (VATs) 3 and 7. The 7 ELC has a restriction map upstream from the 5' barren region that differs from, but is similar to, that of the 3 ELC. These data indicate that the 3 and 7 ELCs are on different telomeres when expressed.

MeSH Terms
Animals Chromosomes/physiology Cloning, Molecular DNA/analysis DNA Restriction Enzymes Epitopes/analysis Genes Genetic Linkage Genetic Variation Glycoproteins/genetics,immunology Nucleic Acid Hybridization Plasmids Species Specificity Trypanosoma brucei brucei/genetics Variant Surface Glycoproteins, Trypanosoma
Chemicals
Epitopes Glycoproteins Variant Surface Glycoproteins, Trypanosoma DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Allison J
Rothwell V
Newport G
Agabian N
Stuart K
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.23.9051
Published
1984-12-11
Pages
9051-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320437
Subset
IM
Grants
NIAID NIH HHS · AI 17309 · United States
NIAID NIH HHS · AI 17375 · United States
External Links
PubMed source DOI: 10.1093/nar/12.23.9051 PMC Full Text View journal details
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