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PMID: 6843549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA rearrangements and antigenic variation in Trypanosoma equiperdum: multiple expression-linked sites in independent isolates of trypanosomes expressing the same antigen.

Molecular and cellular biology ·Vol. 3 ·No. 3 ·1983-03-00 ·Pages 399-409

Longacre S, Hibner U, Raibaud A, Eisen H, Baltz T, Giroud C, Baltz D

Abstract

African trypanosomes resist the immune response of their mammalian hosts by varying the surface glycoprotein which constitutes their antigenic identity. The molecular mechanism of this antigenic variation involves the successive activation of a series of genes which code for different variant surface glycoproteins (VSGs). We have studied the expression of two VSG genes (those of VSG-1 and VSG-28) in Trypanosoma equiperdum, and we report the following findings. (i) The expression of both VSG genes is associated with the duplication and transposition of corresponding basic copy genes. (ii) The duplicated transposed copy appears to be the expressed copy. (iii) Although there are multiple genes which cross-hybridize with the VSG-1 cDNA probe, only one of these appears to be used as a template for the expression-linked copy in four independent BoTat-1 clones. (iv) Analysis of the genomic environments of the expressed VSG-1 genes from each of four independently derived BoTat-1 trypanosome clones revealed that there are at least three different sites into which the expression-linked copy can be inserted.

MeSH Terms
Animals Antibody Formation Antigens/genetics Cloning, Molecular DNA/analysis Gene Expression Regulation Glycoproteins/genetics Plasmids Trypanosoma/genetics,immunology Variant Surface Glycoproteins, Trypanosoma
Chemicals
Antigens Glycoproteins Variant Surface Glycoproteins, Trypanosoma DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Longacre S
Hibner U
Raibaud A
Eisen H
Baltz T
Giroud C
Baltz D
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.3.399-409.1983
Published
1983-03-00
Pages
399-409
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368548
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.3.3.399-409.1983 PMC Full Text View journal details
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