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PMID: 6278423 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the DNA duplication-transposition that controls the expression of two genes for variant surface glycoproteins in Trypanosoma brucei.

Nucleic acids research ·Vol. 10 ·No. 2 ·1982-01-22 ·Pages 593-609

Van der Ploeg LH, Bernards A, Rijsewijk FA, Borst P

Abstract

The genome of Trypanosoma brucei carries over a hundred genes coding for different variants of the major surface glycoprotein. Activation of some of these genes is accompanied by a duplication and transposition of the gene (the basic copy) to another region in the genome where it is transcribed. We present here physical maps of the basic and transposition-activated genes for two surface glycoproteins of Trypanosoma brucei, stock 427. In both cases the transposed segment starts 1-2 kb in front of the coding region and ends within the 3'-terminal region of the gene. The DNA segments flanking both transposed genes are indistinguishable and share a 6-kb stretch upstream and a 8-kb stretch downstream of the transposed segment not cut by several restriction endonucleases. The 5' borders of the two transposed segments are homologous and contain sequences present in many copies in the genome. A different repeated sequence has previously been found at the 3' edge of the transposed segment. The replicative transposition may, therefore, involve a unidirectional gene conversion initiated by base pairing between the edges of the transposed sequence and a single expression site elsewhere in the genome.

MeSH Terms
Animals Cell Nucleus/metabolism Cloning, Molecular DNA/metabolism DNA Replication DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Glycoproteins/genetics Membrane Proteins/genetics Nucleic Acid Hybridization Transcription, Genetic Trypanosoma brucei brucei/metabolism
Chemicals
DNA, Recombinant Glycoproteins Membrane Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Van der Ploeg L H
Bernards A
Rijsewijk F A
Borst P
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40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.2.593
Published
1982-01-22
Pages
593-609
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326160
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.2.593 PMC Full Text View journal details
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