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PMID: 6170939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Homologous 3'-terminal regions of mRNAs for surface antigens of different antigenic variants of Trypanosoma brucei.

Nucleic acids research ·Vol. 9 ·No. 18 ·1981-09-25 ·Pages 4745-53

Majumder HK, Boothroyd JC, Weber H

Abstract

Sequences corresponding to the complete 3'-terminal regions of the messenger RNAs for three different Variant Surface Glycoproteins of Trypanosoma brucei were determined on complementary DNA inserts cloned in recombinant plasmids. The three sequences show 80-130 base pair long segments of strong (70-80%) homology at the 3' ends, whereas ther regions upstream from the last 130-140 base pairs contain no significant homology. The signal AAUAAA, present near the 3' ends of almost all known polyadenylated mRNAs of eukaryotes, does not occur in the 3'-terminal sequences of these three variants.

MeSH Terms
Amino Acid Sequence Animals Antigens, Surface/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Epitopes/genetics Genetic Variation Plasmids RNA, Messenger/genetics Trypanosoma brucei brucei/genetics,immunology
Chemicals
Antigens, Surface DNA, Recombinant Epitopes RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Majumder H K
Boothroyd J C
Weber H
References (15)
15 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.18.4745
Published
1981-09-25
Pages
4745-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327472
Subset
IM
Databases
GENBANK
J01229, K00641, K00642, V01387
External Links
PubMed source DOI: 10.1093/nar/9.18.4745 PMC Full Text View journal details
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