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PMID: 6258157 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of DNA sequences complementary to messenger ribonucleic acids coding for the synthesis of two surface antigens of Trypanosoma brucei.

Nucleic acids research ·Vol. 8 ·No. 24 ·1980-12-20 ·Pages 5965-81

Pays E, Delronche M, Lheureux M, Vervoort T, Bloch J, Gannon F, Steinert M

Abstract

Full length double-stranded complementary DNAs (ds cDNAs) could be synthesized on mRNAs enriched in sequences coding for the synthesis of the variant specific antigens (VSAs) AnTat 1.1 and AnTat 1.8 from Trypanosoma brucei brucei. The size of these ds cDNAs is about 1700 and 1850 base pairs for AnTat 1.1 and AnTat 1.8 respectively. The ds cDNAs were cloned in the plasmid pBR322; two clones harboring a copy of each coding sequence were selected. Both the hybrid-arrested translation and the positive hybridization elution methods confirmed that these recombinants contain VSA-specific inserts. A restriction map was constructed in each case. The two sequences seem to be inserted in a reversed 3'--5' orientation, respective to the plasmid polarity. The AnTat 1.8 cloned sequence is a palindrome probably due to a cloning artefact. Hybridization of the cloned DNAs with "Northern" blots of total or poly(A)+ RNA revealed in each case a single, specific band. The expression of these VSA genes appears thus to be regulated at the transcriptional level.

MeSH Terms

Antigens, Surface/genetics Cloning, Molecular DNA/biosynthesis DNA Restriction Enzymes Nucleic Acid Hybridization Plasmids RNA, Messenger/metabolism Templates, Genetic Transcription, Genetic Trypanosoma brucei brucei/genetics,immunology

Chemicals

Antigens, Surface RNA, Messenger DNA DNA Restriction Enzymes

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Pays E

Delronche M

Lheureux M

Vervoort T

Bloch J

Gannon F

Steinert M

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28 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.24.5965

Published

1980-12-20

Pages

5965-81

Language

English

Region

England

NLM ID

0411011

PMCID

PMC328065

Subset

External Links

PubMed source DOI: 10.1093/nar/8.24.5965 PMC Full Text View journal details

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Cloning and characterization of DNA sequences complementary to messenger ribonucleic acids coding for the synthesis of two surface antigens of Trypanosoma brucei.

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