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PMID: 6283477 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the expression-linked gene copies of variant surface glycoprotein 118 in two independently isolated clones of Trypanosoma brucei.

Nucleic acids research ·Vol. 10 ·No. 7 ·1982-04-10 ·Pages 2353-66

Michels PA, Bernards A, Van der Ploeg LH, Borst P

Abstract

We have analysed the gene for variant surface glycoprotein 118 in eight independent clones of Trypanosoma brucei, two of which express the 118 gene. Expression of this gene is strictly coupled to the presence of an extra copy of the gene. In both clones the expression-linked copy is transposed to the same (or a very similar) expression site elsewhere in the genome, but the length of the sequences flanking the transposed segment in the expression site differs markedly. By means of S1 nuclease protection experiments we demonstrate that the 3'-ends of the messenger RNAs for variant surface glycoproteins 118a and 118b are different, in agreement with the hypothesis that the generation of an expression-linked copy involves a recombination between the 3' segment of the basic gene copy and a homologous region present in the expression site.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes Endonucleases Female Genes Genetic Variation Glycoproteins/genetics Mice Nucleic Acid Hybridization Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic Trypanosoma brucei brucei/genetics
Chemicals
Glycoproteins Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Michels P A
Bernards A
Van der Ploeg L H
Borst P
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.7.2353
Published
1982-04-10
Pages
2353-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320614
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.7.2353 PMC Full Text View journal details
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