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PMID: 6866765 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Size fractionation of Trypanosoma brucei DNA: localization of the 177-bp repeat satellite DNA and a variant surface glycoprotein gene in a mini-chromosomal DNA fraction.

Nucleic acids research ·Vol. 11 ·No. 12 ·1983-06-25 ·Pages 3889-901

Sloof P, Menke HH, Caspers MP, Borst P

Abstract

We have size-fractionated intact DNA from Trypanosoma brucei into a major large DNA fraction (greater than 350S) and minor middle-sized (60-250S) and small (less than 60S) DNA fractions. Large DNA contains the rRNA genes, the basic copy genes for several variant surface glycoproteins (VSGs), including one which lies near a telomer, and the expression-linked copies of the two VSG genes. The middle-sized DNA contains at least one VSG gene, but the hybridization of this fraction with probes for the conserved repetitive sequences that mark the edges of the transposed segments of VSG genes, suggests that it may contain many VSG genes. The 177-bp repeat satellite DNA is also exclusively found in this fraction.

MeSH Terms
Animals DNA/analysis Electrophoresis, Agar Gel Glycoproteins/genetics Molecular Weight Nucleic Acid Hybridization Rats Trypanosoma brucei brucei/genetics Variant Surface Glycoproteins, Trypanosoma
Chemicals
Glycoproteins Variant Surface Glycoproteins, Trypanosoma DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sloof P
Menke H H
Caspers M P
Borst P
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.12.3889
Published
1983-06-25
Pages
3889-901
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326014
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.12.3889 PMC Full Text View journal details
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