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PMID: 6091055 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Subtelomeric regions of yeast chromosomes contain a 36 base-pair tandemly repeated sequence.

Nucleic acids research ·Vol. 12 ·No. 18 ·1984-09-25 ·Pages 7105-21

Horowitz H, Haber JE

Abstract

We have determined the nucleotide sequence of a region of DNA derived from the end of one chromosome of the yeast, Saccharomyces cerevisiae. Inspection of the sequence reveals the presence of 12 tandem direct repeats, each 36 nucleotides long and having nearly identical sequence. Each 36 base-pair repeat can be further subdivided into three tandem sub-repeats of a similar 12 base-pair sequence. Analysis of total genomic yeast DNA from several strains by Southern hybridization suggests that the number of tandem 36 base-pair repeat units may vary from approximately 8 to 25 among different telomeric regions. Differences in the number of repeats may have arisen by unequal crossing over between them. Furthermore, the finding that the pattern of bases at multiple variable positions within the repeat unit is not random suggests that these regions may undergo gene conversion events that render them homogeneous.

MeSH Terms

Base Composition Base Sequence Chromosomes/analysis DNA Restriction Enzymes DNA, Fungal/genetics Genes, Fungal Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Species Specificity

Chemicals

DNA, Fungal DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Horowitz H

Haber J E

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27 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.18.7105

Published

1984-09-25

Pages

7105-21

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320145

Subset

Databases

GENBANK

X00971

External Links

PubMed source DOI: 10.1093/nar/12.18.7105 PMC Full Text View journal details

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Subtelomeric regions of yeast chromosomes contain a 36 base-pair tandemly repeated sequence.

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