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PMID: 6364141 Published · ppublish English Journal Article

Primary structure and transcription of an amplified genetic locus: the CUP1 locus of yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 2 ·1984-01-00 ·Pages 337-41

Karin M, Najarian R, Haslinger A, Valenzuela P, Welch J, Fogel S

Abstract

Copper resistance in yeast is controlled by the CUP1 locus. The level of resistance is proportional to the copy number of this locus, which can be found in up to 15 tandemly iterated copies. To elucidate the molecular mechanisms controlling the amplification and expression of the CUP1, locus, we determined its full nucleotide sequence. We have also identified and mapped two transcription units within the basic amplification unit of CUP1 in laboratory yeast strains. One of those transcription units is inducible by copper and encodes a low molecular weight copper binding protein--copper chelatin. The increased production of chelatin, due to both gene amplification and induction of transcription, leads to increased resistance of yeast cells to copper ions.

MeSH Terms
Amino Acid Sequence Base Sequence Carrier Proteins/genetics Chromosome Mapping Copper/pharmacology DNA, Fungal/genetics Drug Resistance, Microbial Gene Amplification Gene Expression Regulation/drug effects Genes Metalloproteins/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic/drug effects
Chemicals
Carrier Proteins DNA, Fungal Metalloproteins chelatin Copper
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Karin M
Najarian R
Haslinger A
Valenzuela P
Welch J
Fogel S
References (37)
37 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. Human metallothionein genes--primary structure of the metallothionein-II gene and a related processed gene.
    Nature. 1982 Oct 28;299(5886):797-802 PMID: 7133118
  3. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.
    J Biol Chem. 1977 Nov 25;252(22):8118-25 PMID: 334774
  4. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  5. Histone genes are clustered with a 15-kilobase repeat in the chicken genome.
    Nature. 1979 May 10;279(5709):132-6 PMID: 440417
  6. The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis.
    J Mol Biol. 1976 Aug 25;105(4):461-86 PMID: 787539
  7. The genes for 18S, 5.8S and 28S ribosomal RNA of Bombyx mori are organized into tandem repeats of uniform length.
    Gene. 1978 Oct;4(2):153-66 PMID: 363521
  8. The structural organization of ribosomal DNA in Drosophila melanogaster.
    Cell. 1977 Feb;10(2):193-212 PMID: 402222
  9. Tandem gene amplification mediates copper resistance in yeast.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5342-6 PMID: 6291039
  10. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  11. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  12. Analysis of the junction between ribosomal RNA genes and single-copy chromosomal sequences in the yeast Saccharomyces cerevisiae.
    Cell. 1982 Feb;28(2):355-64 PMID: 6277511
  13. A structure for amplified DNA.
    Cell. 1983 May;33(1):53-63 PMID: 6678612
  14. A versatile primer for DNA sequencing in the M13mp2 cloning system.
    Gene. 1980 Jun;10(1):69-73 PMID: 6250947
  15. Adjacent repeating units of Xenopus laevis 5S DNA can be heterogeneous in length.
    Cell. 1976 Apr;7(4):477-86 PMID: 782719
  16. Molecular analysis of the histone gene cluster of psammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences.
    Cell. 1976 Aug;8(4):471-8 PMID: 954100
  17. Cuprodoxin.
    FEBS Lett. 1975 Jun 15;54(2):224-9 PMID: 165979
  18. Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA.
    Mol Gen Genet. 1977 Mar 16;151(3):229-44 PMID: 325373
  19. A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA.
    Cell. 1977 Feb;10(2):167-76 PMID: 402220
  20. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  21. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  22. Gene control of coppersensitivity in Saccharomyces.
    Nature. 1955 Oct 29;176(4487):841-2 PMID: 13272707
  23. The organization of genetic units in chromosomes.
    J Cell Sci. 1967 Mar;2(1):1-7 PMID: 6031004
  24. Chromosome Mapping in Saccharomyces: Centromere-Linked Genes.
    Genetics. 1960 Aug;45(8):1085-110 PMID: 17247984
  25. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  26. Expression and regulation of a human metallothionein gene carried on an autonomously replicating shuttle vector.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):4040-4 PMID: 6306660
  27. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  28. Unequal mitotic sister chromatid exchange and disproportionate replication as mechanisms regulating ribosomal RNA gene redundancy.
    Cold Spring Harb Symp Quant Biol. 1974;38:491-500 PMID: 4208791
  29. Amplification as a rectification mechanism for the redundant rRNA genes.
    Nat New Biol. 1972 Aug 2;238(83):134-7 PMID: 4538238
  30. Homologous copper(I)-(thiolate)2-chromophores in yeast copper thionein.
    Biochim Biophys Acta. 1977 Aug 23;493(2):465-77 PMID: 329891
  31. Histone genes of the sea urchin (S. purpuratus) cloned in E coli: order, polarity, and strandedness of the five histone-coding and spacer regions.
    Cell. 1976 Sep;9(1):147-61 PMID: 788917
  32. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  33. Amplification of the metallothionein-I gene in cadmium-resistant mouse cells.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2110-4 PMID: 6941274
  34. Promoter sequences of eukaryotic protein-coding genes.
    Science. 1980 Sep 19;209(4463):1406-14 PMID: 6251548
  35. Copper-chelatin: isolation from various eucaryotic sources.
    Arch Biochem Biophys. 1975 Sep;170(1):278-88 PMID: 169747
  36. Yeast gene TRP5: structure, function, regulation.
    J Biol Chem. 1982 Feb 10;257(3):1491-500 PMID: 6276387
  37. Industrial yeasts display tandem gene iteration at the CUP1 region.
    Mol Cell Biol. 1983 Aug;3(8):1353-61 PMID: 6621529
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.2.337
Published
1984-01-00
Pages
337-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344671
Subset
IM
Databases
GENBANK
K02204
External Links
PubMed source DOI: 10.1073/pnas.81.2.337 PMC Full Text View journal details
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