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PMID: 2988942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties.

The EMBO journal ·Vol. 4 ·No. 5 ·1985-05-00 ·Pages 1279-86

Delmas V, Bastien C, Scherneck S, Feunteun J

Abstract

The hamster papovavirus (HapV) is associated with multiple skin epitheliomas of the Syrian hamster. We have sequenced its genome. It is a double-stranded circular DNA of 5366 bp. The hypothetical genomic organization deduced from this nucleotide sequence is clearly of the polyoma type with the two strands coding in the opposite directions from a noncoding region that shows some of the features of a replication origin and a transcription control region. The amino acid sequences predicted from the open reading frames show an average of 50% homology with polyoma-coded polypeptides. The HapV is, after polyoma, the second example of a papovavirus coding for a middle T antigen. The cloned DNA can immortalize primary rat embryo cells and transform an established rat cell line. The viral DNA is stably integrated into the host genome.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/analysis Base Sequence Cell Transformation, Viral Cricetinae DNA, Viral/analysis Genes, Viral Papillomaviridae/genetics Polyomaviridae Polyomavirus/genetics Rats Rats, Inbred F344 Viral Proteins/analysis
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor DNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Delmas V
Bastien C
Scherneck S
Feunteun J
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23 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-05-00
Pages
1279-86
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554337
Subset
IM
Databases
GENBANK
X02449
External Links
PubMed source PMC Full Text View journal details
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