Home LiteratureArticle Details
PMID: 6280176 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 4 ·1982-02-00 ·Pages 973-7

Patschinsky T, Hunter T, Esch FS, Cooper JA, Sefton BM

Abstract

We have identified the single phosphorylated tyrosine in p60src, the transforming protein of Rous sarcoma virus, as part of the sequence. NH2-Arg-Leu-Ile-Glu-Asp-Asn-Glu-Tyr(P)-Thr-Ala-Arg-COOH. Therefore, this is a sequence that is recognized efficiently by a tyrosine protein kinase in vivo. Phosphorylation of tyrosine in cellular proteins appears to play a role in malignant transformation by four classes of genetically distinct RNA tumor viruses. Phosphorylated tyrosines in several other proteins resemble of the tyrosine in p60src in that they are located 7 residues to the COOH-terminal side of a basic amino acid and either 4 residues to the COOH-terminal side of, or in close proximity to, a glutamic acid residue. Therefore it is possible that these features play a role in the selection of sites of phosphorylation by some tyrosine protein kinases. However, several clear exceptions to this rule exist.

MeSH Terms
Abelson murine leukemia virus Alpharetrovirus Amino Acid Sequence Avian Sarcoma Viruses Cell Transformation, Neoplastic Cell Transformation, Viral Immunoglobulin Heavy Chains Peptides/metabolism Phosphorylation Protein Kinases/metabolism Transforming Growth Factors Tyrosine/metabolism Viral Proteins/metabolism
Chemicals
Immunoglobulin Heavy Chains Peptides Viral Proteins Tyrosine Transforming Growth Factors Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Patschinsky T
Hunter T
Esch F S
Cooper J A
Sefton B M
References (37)
37 references, click to expand
  1. [Primary structure of bovine s1 casein. Complete sequence].
    Eur J Biochem. 1971 Nov 11;23(1):41-51 PMID: 4331376
  2. Staphylococcal protease: a proteolytic enzyme specific for glutamoyl bonds.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3506-9 PMID: 4509307
  3. Direct transformation of 3T3 cells by Abelson murine leukaemia virus.
    Nature. 1975 Feb 27;253(5494):729-31 PMID: 163444
  4. Substrate specificity of the cyclic AMP-dependent protein kinase.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3448-52 PMID: 1059131
  5. Role of multiple basic residues in determining the substrate specificity of cyclic AMP-dependent protein kinase.
    J Biol Chem. 1977 Jul 25;252(14):4888-94 PMID: 194899
  6. Identification of a polypeptide encoded by the avian sarcoma virus src gene.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1567-71 PMID: 206909
  7. Identification of an Abelson murine leukemia virus-encoded protein present in transformed fibroblast and lymphoid cells.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2488-92 PMID: 209468
  8. Cells nonproductively transformed by Abelson murine leukemia virus express a high molecular weight polyprotein containing structural and nonstructural components.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3974-8 PMID: 211510
  9. Characterization of Rous sarcoma virus src gene products synthesized in vitro.
    J Virol. 1978 Nov;28(2):551-66 PMID: 214578
  10. Comparison of the expression of the src gene of Rous sarcoma virus in vitro and in vivo.
    J Virol. 1978 Dec;28(3):957-71 PMID: 215787
  11. Biological properties and translational products of three independent isolates of feline sarcoma virus.
    Virology. 1979 Jan 15;92(1):91-107 PMID: 217170
  12. Cyclic nucleotide-independent protein kinases from rabbit reticulocytes. Site-specific phosphorylation of casein variants.
    Eur J Biochem. 1979 Mar;94(2):497-504 PMID: 218819
  13. Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation.
    J Virol. 1979 Feb;29(2):770-81 PMID: 219258
  14. Transformation by Rous sarcoma virus: effects of src gene expression on the synthesis and phosphorylation of cellular polypeptides.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5212-6 PMID: 228282
  15. Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6260-4 PMID: 230504
  16. Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine.
    Nature. 1980 Feb 28;283(5750):826-31 PMID: 6244493
  17. Temperature-sensitive transformation by Rous sarcoma virus and temperature-sensitive protein kinase activity.
    J Virol. 1980 Jan;33(1):220-9 PMID: 6245228
  18. Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.
    Nature. 1980 May 15;285(5761):167-9 PMID: 6246443
  19. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  20. Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
    J Biol Chem. 1980 Sep 25;255(18):8363-5 PMID: 6157683
  21. Evidence that the phosphorylation of tyrosine is essential for cellular transformation by Rous sarcoma virus.
    Cell. 1980 Jul;20(3):807-16 PMID: 6251974
  22. Nucleotide sequence of an avian sarcoma virus oncogene (src) and proposed amino acid sequence for gene product.
    Nature. 1980 Sep 18;287(5779):198-203 PMID: 6253794
  23. Origin and functional properties of the major gene product of the Snyder-Theilen strain of feline sarcoma virus.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5158-62 PMID: 6254060
  24. The purified product of the transforming gene of avian sarcoma virus phosphorylates tyrosine.
    J Biol Chem. 1980 Dec 25;255(24):11973-80 PMID: 6254988
  25. Characterization of Y73, an avian sarcoma virus: a unique transforming gene and its product, a phosphopolyprotein with protein kinase activity.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6199-203 PMID: 6255480
  26. Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virus.
    Cell. 1980 Dec;22(3):757-65 PMID: 6257396
  27. Tyrosine-specific protein kinase activity associated with p105 of avian sarcoma virus PRCII.
    Virology. 1981 Feb;109(1):223-8 PMID: 6258311
  28. Evidence that the Abelson virus protein functions in vivo as a protein kinase that phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1552-6 PMID: 6262813
  29. Transforming proteins of some feline and avian sarcoma viruses are related structurally and functionally.
    Cell. 1981 Apr;24(1):145-53 PMID: 6263483
  30. Snyder-Theilen feline sarcoma virus P85 contains a single phosphotyrosine acceptor site recognized by its associated protein kinase.
    J Virol. 1981 Jun;38(3):886-94 PMID: 6165840
  31. Amino-terminal sequence analysis of alphavirus polypeptides.
    J Virol. 1981 Jun;38(3):968-72 PMID: 7241669
  32. Homologous tyrosine phosphorylation sites in transformation-specific gene products of distinct avian sarcoma viruses.
    Nature. 1981 Jun 25;291(5817):675-7 PMID: 6264320
  33. Characterization of a 105,000 molecular weight gag-related phosphoprotein from cells transformed by the defective avian sarcoma virus PRCII.
    Virology. 1981 Jan 15;108(1):98-110 PMID: 6267778
  34. Evidence that there exist four classes of RNA tumor viruses which encode proteins with associated tyrosine protein kinase activities.
    J Virol. 1981 Jul;39(1):104-14 PMID: 6268803
  35. Characterization of sites for tyrosine phosphorylation in the transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homologue (pp60c-src).
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6013-7 PMID: 6273838
  36. Changes in protein phosphorylation in Rous sarcoma virus-transformed chicken embryo cells.
    Mol Cell Biol. 1981 Feb;1(2):165-78 PMID: 6100962
  37. Four different classes of retroviruses induce phosphorylation of tyrosines present in similar cellular proteins.
    Mol Cell Biol. 1981 May;1(5):394-407 PMID: 6086011
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.4.973
Published
1982-02-00
Pages
973-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345881
Subset
IM
Grants
NCI NIH HHS · CA 14195 · United States
NCI NIH HHS · CA 17096 · United States
NCI NIH HHS · CA 17289 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.4.973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2