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PMID: 2117703 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML.

Molecular and cellular biology ·Vol. 10 ·No. 9 ·1990-09-00 ·Pages 4932-4

Park EC, Szostak JW

Abstract

The N-terminal serine and four conserved lysine residues near the N-terminus of yeast histone H4 are acetylated. We found that a mutation that changed the fourth lysine to alanine resulted in specific derepression of the silent mating type locus HML, while mutations that altered the N-terminal serine or the first three lysines had only minor phenotypic effects. Our results support an active role for histone H4 in the silencing of gene expression at this locus.

MeSH Terms
Amino Acid Sequence Chromosomes, Fungal Genes, Fungal Histones/genetics Lysine Molecular Sequence Data Mutation Phenotype Plasmids Saccharomyces cerevisiae/genetics,physiology Serine Spores, Fungal/physiology
Chemicals
Histones Serine Lysine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Park E C
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Szostak J W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.9.4932-4934.1990
Published
1990-09-00
Pages
4932-4
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361112
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.9.4932-4934.1990 PMC Full Text View journal details
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