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PMID: 1922073 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 11 ·No. 11 ·1991-11-00 ·Pages 5710-7

Malone EA, Clark CD, Chiang A, Winston F

Abstract

SPT16 was previously identified as a high-copy-number suppressor of delta insertion mutations in the 5' regions of the HIS4 and LYS2 genes of Saccharomyces cerevisiae. We have constructed null mutations in the SPT16 gene and have demonstrated that it is essential for growth. Temperature-sensitive-lethality spt16 alleles have been isolated and shown to be pleiotropic; at a temperature permissive for growth, spt16 mutations suppress delta insertion mutations, a deletion of the SUC2 upstream activating sequence, and mutations in trans-acting genes required for both SUC2 and Ty expression. In addition, SPT16 is identical to CDC68, a gene previously shown to be required for passage through the cell cycle control point START. However, at least some transcriptional effects caused by spt16 mutations are independent of arrest at START. These results and those in the accompanying paper (A. Rowley, R. A. Singer, and G. C. Johnston, Mol. Cell. Biol. 11:5718-5726, 1991) indicate that SPT16/CDC68 is required for normal transcription of many loci in S. cerevisiae.

Related Genes
his4 lys2 spt16
MeSH Terms
DNA, Fungal/genetics,isolation & purification Genes, Fungal Genotype Mutagenesis, Insertional Plasmids Promoter Regions, Genetic Restriction Mapping Saccharomyces cerevisiae/genetics Suppression, Genetic Transcription, Genetic
Chemicals
DNA, Fungal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Malone E A
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Clark C D
Chiang A
Winston F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.11.5710-5717.1991
Published
1991-11-00
Pages
5710-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361942
Subset
IM
Grants
NIGMS NIH HHS · GM32967 · United States
NIGMS NIH HHS · T32-GM07196 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.11.5710-5717.1991 PMC Full Text View journal details
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