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PMID: 3127692 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SPT3 is required for normal levels of a-factor and alpha-factor expression in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 8 ·No. 2 ·1988-02-00 ·Pages 822-7

Hirschhorn JN, Winston F

Abstract

Mutations in the Saccharomyces cerevisiae SPT3 gene were previously found to cause suppression of Ty and delta insertion mutations in 5'-noncoding regions of genes. This suppression likely results from the fact that SPT3 is required for transcription initiation in delta sequences. Other additional phenotypes of spt3 mutants, including a mating defect, suggest that SPT3 is required for normal levels of expression of other genes. We analyzed the mating defect in spt3 mutants and showed that the levels of transcripts of the three major mating pheromone genes, MF alpha 1, MFa1, MFa2, were all reduced. The reduction in expression of these genes in spt3 mutants was not due to expression of a silent mating type cassette. Furthermore, we showed that the spt3 mating defect was manifest at the levels of both cellular fusion and nuclear fusion.

MeSH Terms
Genes Genes, Fungal Genes, Mating Type, Fungal Genotype Mating Factor Mutation Peptides/genetics Plasmids Saccharomyces cerevisiae/genetics Suppression, Genetic Transcription, Genetic beta-Galactosidase/genetics
Chemicals
Peptides Mating Factor beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hirschhorn J N
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Winston F
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32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.2.822-827.1988
Published
1988-02-00
Pages
822-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363210
Subset
IM
Grants
NIGMS NIH HHS · GM32967 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.2.822-827.1988 PMC Full Text View journal details
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