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PMID: 6344075 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 10 ·1983-05-00 ·Pages 3035-9

Jensen R, Sprague GF, Herskowitz I

Abstract

The ultimate product of yeast mating-type interconversion is a stable a/alpha diploid cell. A haploid cell carrying the HO gene gives rise to a diploid cell in a two-step process: first, the cell switches mating type as a result of genetic rearrangement (cassette substitution) catalyzed by HO; then, cells of opposite type mate to form a/alpha diploids. Mating-type interconversion does not occur in a/alpha diploids despite the presence of the HO gene. We have identified a plasmid carrying the HO gene by screening a yeast clone bank (constructed in vector YEp13) for plasmids that allow mating-type switching by ho cells. The yeast segment responsible for mating-type interconversion integrates by homology at the ho locus, thus confirming that it carries HO. Using the HO gene as a probe, we find that strains with an active mating-type interconversion system produce HO RNA, whereas a/alpha HO/HO cells do not and that this inhibition requires products of both the MATa1 and MATa2 genes. Thus, mating-type interconversion does not occur in a/alpha HO/HO cells because the HO gene product is not synthesized. These results demonstrate the following: (i) The mating-type locus, proposed on genetic grounds to be a regulatory locus, controls expression of an unlinked gene (HO) at the level of RNA production. (ii) The HO gene is under negative feedback control: its expression is inhibited after successful completion of diploidization (formation of a/alpha diploids).

MeSH Terms
DNA, Fungal/genetics Diploidy Feedback Gene Expression Regulation Heterozygote RNA, Fungal/genetics Recombination, Genetic Reproduction Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal RNA, Fungal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jensen R
Sprague G F
Herskowitz I
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.10.3035
Published
1983-05-00
Pages
3035-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393968
Subset
IM
Grants
NIAID NIH HHS · AI-00163 · United States
NIAID NIH HHS · AI-13462 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.10.3035 PMC Full Text View journal details
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