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PMID: 3020500 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of the yeast SPT3 gene and identification of its product, a positive regulator of Ty transcription.

Nucleic acids research ·Vol. 14 ·No. 17 ·1986-09-11 ·Pages 6885-900

Winston F, Minehart PL

Abstract

Previous work has demonstrated that the yeast SPT3 gene is required for transcription from delta sequences, the long terminal repeats that flank yeast Ty elements. In spt3 null mutants, transcription fails to initiate in delta sequences and instead initiates farther downstream. Null mutations in SPT3 cause other mutant phenotypes, including defects in sporulation and diploid formation. In this paper we report further genetic and physical characterization of the SPT3 gene and protein. By extensive linker insertion mutagenesis, we have delimited the region necessary for SPT3 function. From DNA sequence analysis, SPT3 encodes a protein of 337 amino acids. We have identified this protein with an anti-SPT3 antibody. Finally, we show that overproduction of the SPT3 gene product does not alter the level of Ty transcription.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Transposable Elements Fungal Proteins/genetics Gene Expression Regulation Genes, Fungal Mutation RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Transcription Factors/genetics
Chemicals
DNA Transposable Elements Fungal Proteins RNA, Messenger Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Winston F
Minehart P L
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47 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.17.6885
Published
1986-09-11
Pages
6885-900
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311706
Subset
IM
Grants
NIGMS NIH HHS · GM32967 · United States
Databases
GENBANK
X04383
External Links
PubMed source DOI: 10.1093/nar/14.17.6885 PMC Full Text View journal details
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