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PMID: 3001735 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular characterization of the Drosophila vermilion locus and its suppressible alleles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 2 ·1986-01-00 ·Pages 404-8

Searles LL, Voelker RA

Abstract

We have cloned vermilion (v), one of the genes required for brown eye pigment synthesis in Drosophila, using a mutant (vH2a) "tagged" with the transposon P factor. Mutations that disrupt v gene expression are clustered within approximately 2 kilobases of DNA. A 1.4-kilobase transcript, homologous to this same region, is present in v+ RNA but absent in RNA from several v mutants. The spontaneous v alleles that are suppressed by the suppressor of sable [su(s)] are apparently identical insertions of 412, a copia-like transposable element. Preliminary evidence suggests that su(s)-suppressible alleles at other loci may also be 412 insertions.

MeSH Terms
Alleles Animals Chromosome Mapping Cloning, Molecular DNA Transposable Elements Drosophila melanogaster/genetics Eye Genes, Regulator Mutation Nucleic Acid Hybridization Pigmentation Transcription, Genetic
Chemicals
DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Searles L L
Voelker R A
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.2.404
Published
1986-01-00
Pages
404-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322867
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.2.404 PMC Full Text View journal details
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