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PMID: 6189122 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 9 ·1983-05-00 ·Pages 2432-6

Elder RT, Loh EY, Davis RW

Abstract

The RNA homologous to the yeast transposable element Ty1 is one of the more abundant poly(A)+ RNAs in many strains of the yeast Saccharomyces cerevisiae. The 5' and 3' ends of Ty1 RNA have been determined from analysis of cDNA. The 5' end is 245 bases into the left delta sequence measured from the left side of the Ty1 element. The delta sequence is a direct repeat of about 340 base pairs present at each end of the Ty1 element. The Ty1 transcription includes 93-97 bases of the left delta sequence and continues through the entire internal portion of the element and through about 295 bases of the right delta sequence before reaching the 3' end located 38-46 bases from the right side of the right delta sequence. Because the delta sequences present at each end of a single Ty1 element have identical or very similar DNA sequences, these end points for Ty1 RNA raise several questions about the expression of Ty1 elements. First, what are the initiation and termination signals, because the Ty1 transcript must read through a DNA sequence that is identical to the 3' end at about 50 bases from the 5' end? Second, why is the direction of transcription of the Ty1 element opposite to that of genes that are overexpressed after the insertion of a Ty1 element? Third, because the Ty1 RNA itself has direct repeats of about 45 bases, a structure analogous to retrovirus RNAs, is the Ty1 RNA an intermediate in the transposition of Ty1?

MeSH Terms
Base Sequence DNA/analysis DNA Transposable Elements Nucleic Acid Conformation Nucleic Acid Hybridization Poly A/analysis RNA/analysis RNA, Messenger Saccharomyces cerevisiae/genetics
Chemicals
DNA Transposable Elements RNA, Messenger Poly A RNA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Elder R T
Loh E Y
Davis R W
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.9.2432
Published
1983-05-00
Pages
2432-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393839
Subset
IM
Grants
NIGMS NIH HHS · GM-21891 · United States
NCRR NIH HHS · S07 RR-05367-19 · United States
Databases
GENBANK
K00948, K00949, K00950, K00951, K00952
External Links
PubMed source DOI: 10.1073/pnas.80.9.2432 PMC Full Text View journal details
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