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PMID: 2997719 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast.

Nucleic acids research ·Vol. 13 ·No. 18 ·1985-09-25 ·Pages 6679-93

Warmington JR, Waring RB, Newlon CS, Indge KJ, Oliver SG

Abstract

We have determined the nucleotide sequence of a class II yeast transposon (Ty 1-17) which is found just centromere-distal to the LEU2 structural gene on chromosome III of Saccharomyces cerevisiae. The complete element is 5961 bp long and is bounded by two identical, directly repeated, delta sequences of 332 bp each. The sequence organization indicates that Ty 1-17 is a retrotransposon, like the class I elements characterized previously. It contains two long open reading-frames, TyA (439 amino acids) and TyB (1349 amino acids). In this paper, the sequences of the two classes of yeast transposon are compared with one another and with analogous elements, such as retroviral proviruses, cauliflower mosaic virus and copia sequences. Features of the Ty 1-17 sequence which may be important to its mechanism of transposition and its genetic action are discussed.

MeSH Terms
Amino Acid Sequence Base Sequence Centromere/ultrastructure Chromosome Mapping Codon DNA Restriction Enzymes DNA Transposable Elements Models, Genetic Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Codon DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Warmington J R
Waring R B
Newlon C S
Indge K J
Oliver S G
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.18.6679
Published
1985-09-25
Pages
6679-93
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321985
Subset
IM
Grants
NIGMS NIH HHS · GM21510 · United States
Databases
GENBANK
M19542, X02991, X03840
External Links
PubMed source DOI: 10.1093/nar/13.18.6679 PMC Full Text View journal details
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