Abstract
A comparative analysis of a number of yeast DNA-pBR322 recombinant plasmids carrying repetitive sequence elements has revealed that Ty1 or delta elements occur in the vicinity of several tRNA genes. Four examples have been characterized in detail: three glutamate tRNA genes and a serine tRNA gene. The tRNAGlu3 genes occupy different chromosomal locations; two of these genes are found adjacent to Ty1 elements, and the third is found adjacent to an independent delta element. A delta unit is also found adjacent to a tRNASer2 gene. Next to one of the tRNAGlu3 genes, the delta element is joined to a truncated sigma element. Junctions between different delta units were characterized by the sequence analysis of two DNA segments that carry no tRNA genes.
MeSH Terms
Base Sequence
DNA Restriction Enzymes
DNA Transposable Elements
DNA, Fungal/genetics
DNA, Recombinant/analysis
Genes, Fungal
Nucleic Acid Hybridization
Plasmids
RNA, Transfer/genetics
Repetitive Sequences, Nucleic Acid
Saccharomyces cerevisiae/genetics
Chemicals
DNA Transposable Elements
DNA, Fungal
DNA, Recombinant
RNA, Transfer
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Eigel A
Feldmann H
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