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PMID: 2685570 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription.

Molecular and cellular biology ·Vol. 9 ·No. 12 ·1989-12-00 ·Pages 5602-9

Fassler JS, Winston F

Abstract

To understand the function of SPT13, a gene encoding a trans-acting factor involved in regulation of Ty-mediated gene expression, we have cloned and sequenced the gene. Our analysis revealed that SPT13 is the same gene as GAL11, a gene in which mutations cause reduced expression of some GAL4-regulated genes. Further analysis of spt13/gal11 mutants suggested that the SPT13 gene also affects transcription of genes involved in mating-type specialization. We show here that SPT13 has both positive and negative regulatory roles in transcription.

MeSH Terms
Blotting, Northern Cloning, Molecular/methods Gene Expression Regulation, Fungal Genes, Fungal Genes, Regulator Genotype Mating Factor Mutation Nucleic Acid Hybridization Peptides/genetics Plasmids RNA, Fungal/genetics,isolation & purification Saccharomyces cerevisiae/genetics,metabolism Trans-Activators/genetics Transcription, Genetic
Chemicals
Peptides RNA, Fungal Trans-Activators Mating Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fassler J S
Department of Biology, University of Iowa, Iowa City 52242.
Winston F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.12.5602-5609.1989
Published
1989-12-00
Pages
5602-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363730
Subset
IM
Grants
NIGMS NIH HHS · GM32967 · United States
NIGMS NIH HHS · GM40306 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.12.5602-5609.1989 PMC Full Text View journal details
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