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PMID: 2838817 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Upstream and downstream transcriptional control signals in the yeast retrotransposon, TY.

Nucleic acids research ·Vol. 16 ·No. 12 ·1988-06-24 ·Pages 5439-58

Fulton AM, Rathjen PD, Kingsman SM, Kingsman AJ

Abstract

The yeast retrotransposon, Ty, shares many structural and functional features with retroviral proviruses. These include production of a terminally redundant major transcript. There are also two less abundant transcripts of 5.0 kb and 2.2 kb. Ty transcription is regulated by cell-type, that is it is reduced 5-20 fold in a/alpha diploids as compared to haploids. However control of expression of Ty is not well understood. By deletion analysis we have identified regions of the element which are involved in the activation and regulation of transcription. These signals are found both upstream and downstream of the mRNA start site. The downstream signals are within the region encoding the major Ty proteins. This organisation of transcriptional control signals is discussed with reference to the organisation of control signals in other yeast genes and in retroviral proviruses and other retro-elements.

MeSH Terms
Base Sequence Chromosome Deletion DNA Transposable Elements Escherichia coli/genetics Gene Expression Regulation Genes, Fungal Molecular Sequence Data Plasmids Protein Biosynthesis RNA, Fungal/genetics,isolation & purification Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements RNA, Fungal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fulton A M
Department of Biochemistry, University of Oxford, UK.
Rathjen P D
Kingsman S M
Kingsman A J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.12.5439
Published
1988-06-24
Pages
5439-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336777
Subset
IM
Databases
GENBANK
X12368
External Links
PubMed source DOI: 10.1093/nar/16.12.5439 PMC Full Text View journal details
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