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PMID: 3031464 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Ty1 sequence with enhancer and mating-type-dependent regulatory activities.

Molecular and cellular biology ·Vol. 7 ·No. 1 ·1987-01-00 ·Pages 258-65

Errede B, Company M, Hutchison CA

Abstract

Some insertion mutations in Saccharomyces cerevisiae activate the expression of adjacent structural genes. The CYC7-H2 mutation is a Ty1 insertion 5' to the iso-2-cytochrome c coding region of CYC7. The Ty1 insertion causes a 20-fold increase in CYC7 expression in a and alpha haploid cell types of S. cerevisiae. This activation is repressed in the a/alpha diploid cell type. Previous computer analysis of the CYC7-H2 Ty1 activator region identified two related sequences with homology both to mammalian enhancers and to a yeast a/alpha control site. A 112-base-pair (bp) DNA fragment encompassing one of these blocks of homology functioned as one component of the Ty1 activator. A 28-bp synthetic oligonucleotide with the wild-type homology block sequence was also functional. A single base pair mutation within the enhancer core of the synthetic 28-bp regulatory element reduced its activation ability to near background amounts. In addition, the 112-bp Ty1 fragment by itself functioned as a target for repression of adjacent gene expression in a/alpha diploid cells.

MeSH Terms
Amino Acid Sequence Base Sequence Cytochrome c Group/genetics Cytochromes c DNA Transposable Elements Diploidy Enhancer Elements, Genetic Genes Genes, Fungal Genes, Mating Type, Fungal Genes, Regulator Mutation Nucleic Acid Hybridization Plasmids Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Cytochrome c Group DNA Transposable Elements iso-2-cytochrome C Cytochromes c
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Errede B
Company M
Hutchison C A
References (53)
53 references, click to expand
  1. Genetic determination of iso-cytochromes c in yeast.
    J Mol Biol. 1965 Aug;13(1):21-39 PMID: 5859036
  2. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  4. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  5. Transformation in Escherichia coli: cryogenic preservation of competent cells.
    J Bacteriol. 1977 Oct;132(1):349-51 PMID: 334731
  6. Evidence for transposition of dispersed repetitive DNA families in yeast.
    Cell. 1979 Apr;16(4):739-51 PMID: 378399
  7. Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 PMID: 7001447
  8. Dependence on mating type for the overproduction of iso-2-cytochrome c in the yeast mutant CYC7-H2.
    Genetics. 1980 Apr;94(4):891-8 PMID: 6254832
  9. Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae.
    Nucleic Acids Res. 1980 Mar 11;8(5):1043-59 PMID: 7003553
  10. Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes.
    Cell. 1980 Nov;22(2 Pt 2):427-36 PMID: 6256080
  11. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.
    Anal Biochem. 1981 Apr;112(2):295-8 PMID: 6266279
  12. Studies on the transposable element Ty1 of yeast. I. RNA homologous to Ty1. II. Recombination and expression of Ty1 and adjacent sequences.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:581-91 PMID: 6266752
  13. Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase.
    Anal Biochem. 1981 May 1;113(1):34-42 PMID: 6168210
  14. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  15. The cyc1-11 mutation in yeast reverts by recombination with a nonallelic gene: composite genes determining the iso-cytochromes c.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6334-8 PMID: 6273865
  16. The molecular characterization of three transcriptional mutations in the yeast iso-2-cytochrome c gene.
    J Biol Chem. 1982 Jul 10;257(13):7756-61 PMID: 6282853
  17. Nucleotide sequence comparisons and functional analysis of yeast centromere DNAs.
    Cell. 1982 May;29(1):235-44 PMID: 7049398
  18. Reversion of a promoter deletion in yeast.
    Nature. 1982 Aug 26;298(5877):815-9 PMID: 6287274
  19. Multiple point mutations affecting the simian virus 40 enhancer.
    Science. 1983 Feb 11;219(4585):626-31 PMID: 6297005
  20. Characterization of transposable element-associated mutations that alter yeast alcohol dehydrogenase II expression.
    Mol Cell Biol. 1983 Jan;3(1):20-31 PMID: 6298605
  21. RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2432-6 PMID: 6189122
  22. Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3035-9 PMID: 6344075
  23. Transposable elements in yeast.
    Int Rev Cytol. 1983;83:1-25 PMID: 6315625
  24. A lymphocyte-specific enhancer in the mouse immunoglobulin kappa gene.
    Nature. 1984 Jan 5-11;307(5946):80-2 PMID: 6419128
  25. Magic enhancers?
    DNA. 1984;3(1):1-5 PMID: 6321119
  26. Linker tailing: unphosphorylated linker oligonucleotides for joining DNA termini.
    DNA. 1984;3(2):173-82 PMID: 6327214
  27. Molecular cloning, DNA structure, and RNA analysis of the arginase gene in Saccharomyces cerevisiae. A study of cis-dominant regulatory mutations.
    EMBO J. 1982;1(9):1125-31 PMID: 6329729
  28. Expression of the ROAM mutations in Saccharomyces cerevisiae: involvement of trans-acting regulatory elements and relation with the Ty1 transcription.
    EMBO J. 1982;1(9):1133-9 PMID: 6145588
  29. Transcription and regulatory signals at the mating type locus in yeast.
    Cell. 1984 Jul;37(3):969-78 PMID: 6378388
  30. Cell type-specific enhancer element associated with a mouse MHC gene, E beta.
    Nature. 1984 Aug 16-22;310(5978):594-7 PMID: 6087163
  31. Differential regulation of the duplicated isocytochrome c genes in yeast.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4475-9 PMID: 6087325
  32. A positive regulatory site and a negative regulatory site control the expression of the Saccharomyces cerevisiae CYC7 gene.
    Mol Cell Biol. 1984 Oct;4(10):2023-30 PMID: 6095036
  33. Identification of regulatory regions within the Ty1 transposable element that regulate iso-2-cytochrome c production in the CYC7-H2 yeast mutant.
    Mol Cell Biol. 1984 Jul;4(7):1393-401 PMID: 6095068
  34. Ty elements transpose through an RNA intermediate.
    Cell. 1985 Mar;40(3):491-500 PMID: 2982495
  35. Identification and comparison of two sequence elements that confer cell-type specific transcription in yeast.
    Nature. 1985 Apr 18-24;314(6012):598-603 PMID: 3887184
  36. Nucleotide sequence of a yeast Ty element: evidence for an unusual mechanism of gene expression.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2829-33 PMID: 2581255
  37. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  38. Activation regions in a yeast transposon have homology to mating type control sequences and to mammalian enhancers.
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5423-7 PMID: 2991922
  39. Transposable element sequences involved in the enhancement of yeast gene expression.
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5428-32 PMID: 2991923
  40. Ty element transposition: reverse transcriptase and virus-like particles.
    Cell. 1985 Sep;42(2):507-17 PMID: 2411424
  41. Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast.
    Nucleic Acids Res. 1985 Sep 25;13(18):6679-93 PMID: 2997719
  42. Reverse transcriptase activity and Ty RNA are associated with virus-like particles in yeast.
    Nature. 1985 Dec 12-18;318(6046):583-6 PMID: 2415827
  43. A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):710-4 PMID: 3003746
  44. Activation of meiosis and sporulation by repression of the RME1 product in yeast.
    Nature. 1986 Feb 27-Mar 5;319(6056):738-42 PMID: 3513021
  45. The SV40 enhancer is composed of multiple functional elements that can compensate for one another.
    Cell. 1986 May 9;45(3):461-70 PMID: 3009027
  46. Identification of the DNA sequences controlling the expression of the MAT alpha locus of yeast.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2320-4 PMID: 3517864
  47. Point mutations implicate repeated sequences as essential elements of the CYC7 negative upstream site in Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Nov;5(11):2951-8 PMID: 3018489
  48. An anomalous Ty1 structure attributed to an error in reverse transcription.
    Mol Cell Biol. 1986 Apr;6(4):1334-8 PMID: 3023883
  49. Transcriptional analysis of Ty1 deletion and inversion derivatives at CYC7.
    Mol Cell Biol. 1986 Oct;6(10):3299-311 PMID: 3025585
  50. Structure of the Saccharomyces cerevisiae HO gene and analysis of its upstream regulatory region.
    Mol Cell Biol. 1986 Dec;6(12):4281-94 PMID: 3025649
  51. SPECTRA OF CYTOCHROME C MONOMER AND POLYMERS.
    Biochim Biophys Acta. 1963 Aug 6;73:641-3 PMID: 14074138
  52. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY.
    Biochim Biophys Acta. 1964 Jul 15;90:1-15 PMID: 14201165
  53. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.1.258-265.1987
Published
1987-01-00
Pages
258-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365065
Subset
IM
Grants
NIAID NIH HHS · AI-08998 · United States
NIGMS NIH HHS · GM-30619 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.1.258-265.1987 PMC Full Text View journal details
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