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PMID: 6095036 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A positive regulatory site and a negative regulatory site control the expression of the Saccharomyces cerevisiae CYC7 gene.

Molecular and cellular biology ·Vol. 4 ·No. 10 ·1984-10-00 ·Pages 2023-30

Wright CF, Zitomer RS

Abstract

A series of BAL31 deletions were constructed in vitro in the upstream region of the Saccharomyces cerevisiae CYC7 gene, encoding the iso-2-cytochrome c protein. These deletions identified two sites which play a role in governing the expression of this gene. A positive site, the deletion of which led to decreased CYC7 expression, lay ca. 240 base pairs 5' to the translational initiation codon (-240). A negative site, the deletion of which led to greatly increased levels of CYC7 expression, lay at ca. -300 bp. Deletion of both these sites resulted in low wild-type-like expression of the gene. Therefore, these two sites appear to act antagonistically to give the low wild-type levels of CYC7 expression. Within the region defined as containing the positive site, there is a sequence which bears some homology to the upstream activation sites in the regulated gene, CYC1, encoding the iso-1-cytochrome c protein.

MeSH Terms
Base Sequence Chromosome Deletion DNA Restriction Enzymes/metabolism Deoxyribonuclease BamHI Deoxyribonucleases, Type II Site-Specific Gene Expression Regulation Plasmids Saccharomyces cerevisiae/genetics
Chemicals
DNA Restriction Enzymes Deoxyribonuclease BamHI CTCGAG-specific type II deoxyribonucleases CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wright C F
Zitomer R S
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30 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.10.2023-2030.1984
Published
1984-10-00
Pages
2023-30
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369019
Subset
IM
Databases
GENBANK
J01320, M17753
External Links
PubMed source DOI: 10.1128/mcb.4.10.2023-2030.1984 PMC Full Text View journal details
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