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PMID: 6244566 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and sequence of the gene for iso-2-cytochrome c in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 1 ·1980-01-00 ·Pages 541-5

Montgomery DL, Leung DW, Smith M, Shalit P, Faye G, Hall BD

Abstract

The two apocytochrome c proteins of yeast are coded for by separate genes. Iso-2-cytochrome c differs from the iso-1 protein at 17 positions within a homologous sequence of 108 amino acids. The previously cloned iso-1-cytochrome c coding sequence has been used to identify lambda-yeast recombinant phage containing the gene for iso-2-cytochrome c. The latter protein contains the dipeptide Ala-Ala which is coded for by the nucleic acid sequence G-C-N-G-C-N. The recognition specificity of restriction endonuclease Fnu4HI for G-C-N-G-C provided a rapid means of locating the region of the cloned fragment which codes for iso-2-cytochrome c. The DNA sequence of this gene has been determined and compared with that of the iso-1-cytochrome c locus. There is no intervening sequence within the gene for iso-2-cytochrome c. At 45 of the 91 positions for which iso-1- and iso-2-cytochrome c have the same amino acid, the codons differ. Such third position variation does not occur within the region coding for amino acids 70-80, the protein sequence that is also most conserved among all eukaryotic cytochromes c.

MeSH Terms
Apoproteins/genetics Base Sequence Codon Cytochrome c Group/genetics DNA Restriction Enzymes DNA, Fungal/genetics DNA, Recombinant Genes RNA, Messenger/genetics Saccharomyces cerevisiae/genetics
Chemicals
Apoproteins Codon Cytochrome c Group DNA, Fungal DNA, Recombinant RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Montgomery D L
Leung D W
Smith M
Shalit P
Faye G
Hall B D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.1.541
Published
1980-01-00
Pages
541-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348308
Subset
IM
Databases
GENBANK
J01320, M17753
External Links
PubMed source DOI: 10.1073/pnas.77.1.541 PMC Full Text View journal details
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