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PMID: 424288 Published · ppublish English Journal Article

A restriction enzyme from Fusobacterium nucleatum 4H which recognizes GCNGC.

Nucleic acids research ·Vol. 6 ·No. 1 ·1979-01-00 ·Pages 17-25

Leung DW, Lui AC, Merilees H, McBride BC, Smith M

Abstract

A site-specific restriction endonuclease Fnu4H I isolated from Fusobacterium nucleatum 4H recognizes the DNA nucleotide sequence 5'G C N G C-3'/3'-C G N C G-5' and cleaves as indicated by the arrows.

MeSH Terms
Base Sequence Coliphages DNA Restriction Enzymes/metabolism DNA, Viral Fusobacterium/enzymology Oligodeoxyribonucleotides/analysis Substrate Specificity
Chemicals
DNA, Viral Oligodeoxyribonucleotides DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Leung D W
Lui A C
Merilees H
McBride B C
Smith M
References (11)
11 references, click to expand
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    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3213-6 PMID: 269384
  2. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  3. A new specific endonuclease present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteum.
    J Mol Biol. 1978 Jan 5;118(1):113-22 PMID: 625054
  4. Computer processing of DNA sequence data.
    J Mol Biol. 1977 Oct 15;116(1):29-30 PMID: 592383
  5. The sequence of a region of bacteriophage phiX174 DNA coding for parts of genes A and B.
    J Mol Biol. 1977 Oct 15;116(1):1-28 PMID: 592379
  6. Nucleotide sequence of bacteriophage phi X174 DNA.
    Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
  7. Restriction endonucleases.
    CRC Crit Rev Biochem. 1976 Nov;4(2):123-64 PMID: 795607
  8. Nucleotide and amino acid sequences of gene G of omegaX174.
    J Mol Biol. 1976 Dec 15;108(3):519-33 PMID: 1088827
  9. A second specific endonuclease from Haemophilus aegyptius.
    J Mol Biol. 1975 Jan 5;91(1):121-3 PMID: 171410
  10. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
    J Mol Biol. 1975 May 25;94(3):441-8 PMID: 1100841
  11. The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.
    FEBS Lett. 1976 Jun 15;65(3):284-7 PMID: 955064
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1979-01-00
Pages
17-25
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327670
Subset
IM
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