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PMID: 6095068 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of regulatory regions within the Ty1 transposable element that regulate iso-2-cytochrome c production in the CYC7-H2 yeast mutant.

Molecular and cellular biology ·Vol. 4 ·No. 7 ·1984-07-00 ·Pages 1393-401

Errede B, Cardillo TS, Teague MA, Sherman F

Abstract

The CYC7-H2 mutation in the yeast Saccharomyces cerevisiae was caused by insertion of a Ty1 transposable element in front of the iso-2-cytochrome c structural gene, CYC7. The Ty1 insertion places iso-2-cytochrome c production under control of regulatory signals that are normally required for mating functions in yeast cells. We have investigated the regions of the Ty1 insertion that are responsible for the aberrant production of iso-2-cytochrome c in the CYC7-H2 mutant. Five alterations of the CYC7-H2 gene were obtained by specific restriction endonuclease cleavage of the cloned DNA and ligation of appropriate fragments. The CYC7+, CYC7-H2, and modified CYC7-H2 genes were each inserted into the yeast vector YIp5 and used to transform a cytochrome c-deficient yeast strain. Expression and regulation of each allele integrated at the CYC7 locus have been compared in vivo by determination of the amount of iso-2-cytochrome c produced. These results show that distal regions of the Ty1 element are not essential for the CYC7-H2 overproducing phenotype. In contrast, alterations in the vicinity of the proximal Ty1 junction abolish the CYC7-H2 expression and give rise to different phenotypes.

MeSH Terms
Base Sequence Crosses, Genetic Cytochrome c Group/analogs & derivatives,genetics Cytochromes c DNA Restriction Enzymes DNA Transposable Elements Genes, Fungal Genes, Regulator Genotype Mutation Plasmids Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Cytochrome c Group DNA Transposable Elements iso-2-cytochrome C Cytochromes c DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Errede B
Cardillo T S
Teague M A
Sherman F
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46 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.7.1393-1401.1984
Published
1984-07-00
Pages
1393-401
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368922
Subset
IM
Grants
NIGMS NIH HHS · R01 GM12702 · United States
NIGMS NIH HHS · R01 GM30619 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.7.1393-1401.1984 PMC Full Text View journal details
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