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PMID: 2821507 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The yeast ROAM mutation--identification of the sequences mediating host gene activation and cell-type control in the yeast retrotransposon, Ty.

Nucleic acids research ·Vol. 15 ·No. 18 ·1987-09-25 ·Pages 7309-24

Rathjen PD, Kingsman AJ, Kingsman SM

Abstract

When the yeast retrotransposon, Ty, integrates into the 5' flanking region of a gene it can activate the expression of that gene. At the same time the activated gene is brought under cell-type specific control such that expression is high in haploid a or alpha cells but low in a/alpha diploids. These Ty mediated mutations are known as ROAM mutations. In this study we have used a ROAM mutation created in vitro to identify the sequences within Ty that mediate this phenomenon. We show that a single activator located within the coding region of the Ty element is responsible for ROAM activation. This sequence, which is regulated by the mating type of the cell, differs from classical enhancer elements in that its activity is strictly orientation dependent. An independent activator located downstream of the ROAM sequence activated transcription only in the non-ROAM orientation. This sequence may be part of an internal promoter that controls expression of the sub-genomic 5.0kb Ty transcript.

MeSH Terms
DNA Transposable Elements DNA, Fungal/genetics Gene Expression Regulation Genes, Fungal Genes, Mating Type, Fungal Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Transcription, Genetic Transcriptional Activation
Chemicals
DNA Transposable Elements DNA, Fungal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rathjen P D
Department of Biochemistry, University of Oxford, UK.
Kingsman A J
Kingsman S M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.18.7309
Published
1987-09-25
Pages
7309-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306250
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.18.7309 PMC Full Text View journal details
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