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PMID: 6329694 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae.

The EMBO journal ·Vol. 1 ·No. 5 ·1982-00-00 ·Pages 603-8

Tuite MF, Dobson MJ, Roberts NA, King RM, Burke DC, Kingsman SM, Kingsman AJ

Abstract

The 5' control region of the yeast phosphoglycerate kinase gene (PGK) was fused to the coding sequence of a human interferon-alpha. This PGK-interferon fusion was then introduced into yeast on a high copy number 2mu-based plasmid vector. Strains containing this plasmid produced a PGK-interferon-alpha fusion protein as 1-2% of cell protein and the expression of interferon activity was regulated by the availability of a fermentable carbon source. The system is capable of making as much as 15 mg of human interferon-alpha per litre of batch culture.

MeSH Terms

Amino Acid Sequence Base Sequence DNA Restriction Enzymes Humans Interferon Type I/genetics,isolation & purification Molecular Weight Phosphoglycerate Kinase/genetics Plasmids Saccharomyces cerevisiae/enzymology,genetics

Chemicals

Interferon Type I Phosphoglycerate Kinase DNA Restriction Enzymes

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Tuite M F

Dobson M J

Roberts N A

King R M

Burke D C

Kingsman S M

Kingsman A J

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28 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1982-00-00

Pages

603-8

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553094

Subset

External Links

PubMed source PMC Full Text View journal details

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Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae.

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