A monoclonal antibody for large-scale purification of human leukocyte interferon.

Nature ·Vol. 285 ·No. 5765 ·1980-06-12 ·Pages 446-50

Secher DS, Burke DC

Abstract

A clone of hybrid myelomas (NK2), secreting a mouse monoclonal antibody to human leukocyte interferon, has been isolated. The antibody neutralizes the antiviral activity of the interferon and, when covalently attached to a solid support and used as an immunoadsorbent, allows interferon purification of up to 5,000-fold in a single step.

MeSH Terms

Antibodies Antibody Specificity Antigen-Antibody Complex Clone Cells/immunology Humans Hybrid Cells/immunology Immunosorbent Techniques Interferons/immunology,isolation & purification Leukocytes/analysis Lymphocytes/analysis Myeloma Proteins/immunology

Chemicals

Antibodies Antigen-Antibody Complex Myeloma Proteins Interferons

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Secher D S

Burke D C

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/285446a0

Published

1980-06-12

Pages

446-50

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/285446a0 View journal details

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A monoclonal antibody for large-scale purification of human leukocyte interferon.

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