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PMID: 6353204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A functional prepro-alpha-factor gene in Saccharomyces yeasts can contain three, four, or five repeats of the mature pheromone sequence.

Molecular and cellular biology ·Vol. 3 ·No. 8 ·1983-08-00 ·Pages 1440-50

Brake AJ, Julius DJ, Thorner J

Abstract

The chromosomal region containing a structural gene for the mating pheromone precursor prepro-alpha-factor was examined in a variety of Saccharomyces yeasts by using a cloned putative prepro-alpha-factor gene of Saccharomyces cerevisiae as the probe. Analysis by restriction endonuclease digestion and Southern blot hybridization indicated that the physical arrangement of this region is highly conserved in all the Saccharomyces species analyzed, but displays length polymorphisms of limited size (50 to 60 base pairs). The observed polymorphisms were shown to be due solely to differences in the number of tandemly arranged spacer peptide/pheromone units within the coding sequence of these genes. Analysis of polyadenylated RNA indicated that these genes specified RNA transcripts and that these RNA molecules could be translated in vitro into prepro-alpha-factor polypeptides immunoprecipitable with anti-alpha-factor antibodies. The sizes of both the mRNAs and the proteins synthesized from them reflected exactly the differences observed in the lengths of the genes. These findings demonstrate conclusively that the putative prepro-alpha-factor DNA cloned from S. cerevisiae, as well as the sequences detected in the other Saccharomyces species, are indeed expressed and functional genes, and suggest that proper proteolytic processing of prepro-alpha-factor is unaffected by the number of pheromone repeats encoded within this precursor protein.

MeSH Terms
Animals Base Sequence DNA, Fungal/genetics Genes Mating Factor Molecular Weight Peptides/genetics Pheromones/genetics Protein Precursors/genetics RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Saccharomyces/genetics Saccharomyces cerevisiae/genetics Species Specificity
Chemicals
DNA, Fungal Peptides Pheromones Protein Precursors RNA, Messenger Mating Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brake A J
Julius D J
Thorner J
References (34)
34 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  3. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
    J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  6. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  7. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
    FEBS Lett. 1977 Oct 15;82(2):314-6 PMID: 913604
  8. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  9. Isolation, characterization, and translation of mRNA from yeast.
    Methods Cell Biol. 1978;20:61-81 PMID: 357927
  10. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  11. Mating pheromones of Saccharomyces kluyveri: pheromone interactions between Saccharomyces kluyveri and Saccharomyces cerevisiae.
    J Bacteriol. 1979 Apr;138(1):146-54 PMID: 374360
  12. Structure of polyadenylic acid in the ribonucleic acid of Saccharomyces cerevisiae.
    J Bacteriol. 1979 May;138(2):542-51 PMID: 374394
  13. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  14. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
    Gene. 1979 Dec;8(1):121-33 PMID: 395029
  15. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
    Gene. 1979 Dec;8(1):17-24 PMID: 395030
  16. Cell-cell recognition in yeast: purification of Hansenula wingei 21-cell sexual agglutination factor and comparison of the factors from three genera.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):318-22 PMID: 6928623
  17. The structure of transposable yeast mating type loci.
    Cell. 1980 Mar;19(3):753-64 PMID: 6244896
  18. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
    Anal Biochem. 1979 Sep 15;98(1):132-5 PMID: 543547
  19. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  20. A versatile primer for DNA sequencing in the M13mp2 cloning system.
    Gene. 1980 Jun;10(1):69-73 PMID: 6250947
  21. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  22. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts.
    Gene. 1980 Oct;11(1-2):11-9 PMID: 7002730
  23. Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2258-62 PMID: 6264471
  24. Control of cell type in yeast by the mating type locus. The alpha 1-alpha 2 hypothesis.
    J Mol Biol. 1981 Apr 15;147(3):357-72 PMID: 7031257
  25. Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA.
    Nature. 1982 Feb 25;295(5851):663-6 PMID: 7057924
  26. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
    J Biol Chem. 1982 Mar 25;257(6):3018-25 PMID: 6277922
  27. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  28. Control of yeast cell type by the mating type locus. II. Genetic interactions between MAT alpha and unlinked alpha-specific STE genes.
    J Mol Biol. 1981 Dec 5;153(2):323-35 PMID: 7040682
  29. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  30. Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.
    Cell. 1982 Oct;30(3):933-43 PMID: 6754095
  31. Phycobilisomes: structure and dynamics.
    Annu Rev Microbiol. 1982;36:173-98 PMID: 6816134
  32. Yeast alpha factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase.
    Cell. 1983 Mar;32(3):839-52 PMID: 6339075
  33. Nucleotide sequence of the yeast SUC2 gene for invertase.
    Nucleic Acids Res. 1983 Mar 25;11(6):1943-54 PMID: 6300785
  34. Structural gene identification utilizing eukaryotic cell-free translational systems.
    Gene Amplif Anal. 1981;2:417-37 PMID: 6985526
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.8.1440-1450.1983
Published
1983-08-00
Pages
1440-50
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369990
Subset
IM
Grants
NIGMS NIH HHS · GM07232 · United States
NIGMS NIH HHS · GM21841 · United States
External Links
PubMed source DOI: 10.1128/mcb.3.8.1440-1450.1983 PMC Full Text View journal details
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