Home LiteratureArticle Details
PMID: 6300785 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the yeast SUC2 gene for invertase.

Nucleic acids research ·Vol. 11 ·No. 6 ·1983-03-25 ·Pages 1943-54

Taussig R, Carlson M

Abstract

The yeast SUC2 gene is a structural gene for both the secreted and intracellular forms of invertase. We have determined the nucleotide sequence of the coding region and the 5' and 3' flanking regions. The coding regions for the signal peptide-containing precursor to secreted invertase and for the intracellular invertase begin at different initiation codons within the SUC2 gene but share the same reading frame. The amino acid sequences predicted for the two forms of invertase from the nucleotide sequence are consistent with the properties of the purified enzymes. Potential sites for glycosylation of the secreted invertase are identified.

MeSH Terms
Amino Acid Sequence Base Sequence Codon/genetics DNA Restriction Enzymes DNA, Fungal/genetics Genes Glycoside Hydrolases/genetics Plasmids Saccharomyces cerevisiae/enzymology,genetics beta-Fructofuranosidase
Chemicals
Codon DNA, Fungal DNA Restriction Enzymes Glycoside Hydrolases beta-Fructofuranosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Taussig R
Carlson M
References (25)
25 references, click to expand
  1. Purification and properties of yeast invertase.
    Biochemistry. 1967 Feb;6(2):468-75 PMID: 4963242
  2. Purification of the internal invertase of yeast.
    J Biol Chem. 1968 Apr 10;243(7):1567-72 PMID: 5647272
  3. Comparative study of the properties of the purified internal and external invertases from yeast.
    J Biol Chem. 1968 Apr 10;243(7):1573-7 PMID: 4967422
  4. The release of intact oligosaccharides from specific glycoproteins by endo-beta-N-acetylglucosaminidase H.
    J Biol Chem. 1974 Feb 10;249(3):818-24 PMID: 4204553
  5. Subunit structure of external invertase from Saccharomyces cerevisiae.
    J Biol Chem. 1977 Jun 25;252(12):4409-12 PMID: 325007
  6. Sequence data handling by computer.
    Nucleic Acids Res. 1977 Nov;4(11):4037-51 PMID: 593900
  7. Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.
    Cell. 1979 Apr;16(4):753-61 PMID: 222467
  8. Carbohydrate structure of yeast invertase. Demonstration of a form with only core oligosaccharides and a form with completed polysaccharide chains.
    J Biol Chem. 1979 Dec 10;254(23):12209-18 PMID: 115881
  9. Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1980 Mar 25;255(6):2596-605 PMID: 6244283
  10. Isolation and sequence of the gene for iso-2-cytochrome c in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):541-5 PMID: 6244566
  11. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  12. Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2546-50 PMID: 6994099
  13. Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.
    Nucleic Acids Res. 1980 May 24;8(10):2255-74 PMID: 6159575
  14. Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 PMID: 7001447
  15. The primary structures of two yeast enolase genes. Homology between the 5' noncoding flanking regions of yeast enolase and glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1981 Feb 10;256(3):1385-95 PMID: 6256394
  16. SUC genes of yeast: a dispersed gene family.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:799-803 PMID: 7021054
  17. Synthesis and processing of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1981;50:555-83 PMID: 7023366
  18. Compartmentalized assembly of oligosaccharides on exported glycoproteins in yeast.
    Cell. 1981 Aug;25(2):451-60 PMID: 7026044
  19. Order of events in the yeast secretory pathway.
    Cell. 1981 Aug;25(2):461-9 PMID: 7026045
  20. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
    J Biol Chem. 1982 Mar 25;257(6):3018-25 PMID: 6277922
  21. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  22. Presecretory and cytoplasmic invertase polypeptides encoded by distinct mRNAs derived from the same structural gene differ by a signal sequence.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):781-5 PMID: 7038684
  23. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  24. Mutants of yeast defective in sucrose utilization.
    Genetics. 1981 May;98(1):25-40 PMID: 7040163
  25. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.6.1943
Published
1983-03-25
Pages
1943-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325848
Subset
IM
Databases
GENBANK
K00540, V01311
External Links
PubMed source DOI: 10.1093/nar/11.6.1943 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2