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PMID: 3275867 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 210-25

Buchman AR, Kimmerly WJ, Rine J, Kornberg RD

Abstract

Two DNA-binding factors from Saccharomyces cerevisiae have been characterized, GRFI (general regulatory factor I) and ABFI (ARS-binding factor I), that recognize specific sequences within diverse genetic elements. GRFI bound to sequences at the negative regulatory elements (silencers) of the silent mating type loci HML E and HMR E and to the upstream activating sequence (UAS) required for transcription of the MAT alpha genes. A putative conserved UAS located at genes involved in translation (RPG box) was also recognized by GRFI. In addition, GRFI bound with high affinity to sequences with the (C1-3A)-repeat region at yeast telomeres. Binding sites for GRFI with the highest affinity appeared to be of the form 5'-(A/G)(A/C)ACCCANNCA(T/C)(T/C)-3', where N is any nucleotide. ABFI-binding sites were located next to autonomously replicating sequences (ARSs) at controlling elements of the silent mating type loci HMR E, HMR I, and HML I and were associated with ARS1, ARS2, and the 2 micron plasmid ARS. Two tandem ABFI binding sites were found between the HIS3 and DED1 genes, several kilobase pairs from any ARS, indicating that ABFI-binding sites are not restricted to ARSs. The sequences recognized by ABFI showed partial dyad-symmetry and appeared to be variations of the consensus 5'-TATCATTNNNNACGA-3'. GRFI and ABFI were both abundant DNA-binding factors and did not appear to be encoded by the SIR genes, whose products are required for repression of the silent mating type loci. Together, these results indicate that both GRFI and ABFI play multiple roles within the cell.

MeSH Terms
Chromosomes/ultrastructure DNA Replication DNA, Fungal/metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation Mating Factor Oligodeoxyribonucleotides/metabolism Peptides/genetics Protein Biosynthesis Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal DNA-Binding Proteins Oligodeoxyribonucleotides Peptides Mating Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Buchman A R
Department of Cell Biology, Stanford University School of Medicine, California 94305.
Kimmerly W J
Rine J
Kornberg R D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.1.210-225.1988
Published
1988-01-00
Pages
210-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363104
Subset
IM
Grants
NIGMS NIH HHS · GM-31105 · United States
NIGMS NIH HHS · GM-36659 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.1.210-225.1988 PMC Full Text View journal details
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