Home LiteratureArticle Details
PMID: 3025597 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Bidirectional promoter elements of simian virus 40 are required for efficient replication of the viral DNA.

Molecular and cellular biology ·Vol. 6 ·No. 10 ·1986-10-00 ·Pages 3513-22

Hertz GZ, Mertz JE

Abstract

Mutants of simian virus 40 (SV40) lacking parts of the 72- and 21-base-pair repeat regions were made deficient in large T antigen by recombination with dlA 4000, a mutant containing a frameshift deletion near the amino terminus of the T antigen genes. These double mutants were transfected into COS cells, and the amounts of replicated viral DNA were measured at various times thereafter. It was found that deletion of either the 72- or 21-base-pair repeat region did not significantly reduce the accumulation of viral DNA. However, cells transfected with mutants lacking both of these promoter elements accumulated 100-fold less viral DNA than cells transfected with wild-type SV40. This indicates that the 72- and 21-base-pair repeat regions are each sufficient for supplying a function required for efficient replication of SV40 DNA. In addition, the ability of either of these regions to support efficient replication was gradually reduced as the number of promoter elements within each was decreased. Since the 72- and 21-base-pair repeat regions bidirectionally induce transcription, our results indicate that bidirectional promoter elements play a role in the replication of viral DNA. However, fewer of these elements are required for efficient replication than for efficient transcription.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics Cell Line DNA Replication DNA Restriction Enzymes DNA, Recombinant/metabolism DNA, Viral/genetics Genes Genes, Viral Mutation Oncogene Proteins, Viral/genetics Promoter Regions, Genetic Simian virus 40/genetics Transfection
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor DNA, Recombinant DNA, Viral Oncogene Proteins, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hertz G Z
Mertz J E
References (56)
56 references, click to expand
  1. Gaps and duplicated sequences in the leaders of SV40 16S RNA.
    Nucleic Acids Res. 1978 Nov;5(11):4195-213 PMID: 82936
  2. Functional organization of the simian virus 40 origin of DNA replication.
    Mol Cell Biol. 1986 Apr;6(4):1117-28 PMID: 3023870
  3. Simian virus 40 early mRNA's. I. Genomic localization of 3' and 5' termini and two major splices in mRNA from transformed and lytically infected cells.
    J Virol. 1979 Apr;30(1):279-96 PMID: 90157
  4. Nucleotide sequence of the Hind-C fragment of simian virus 40 DNA. Comparison of the 5'-untranslated region of wild-type virus and of some deletion Mutants.
    Eur J Biochem. 1979 Oct;100(1):51-60 PMID: 226361
  5. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  6. Regulatory mutants of simian virus 40: constructed mutants with base substitutions at the origin of DNA replication.
    J Mol Biol. 1979 Jul 15;131(4):801-17 PMID: 229233
  7. Cold-sensitive regulatory mutants of simian virus 40.
    J Mol Biol. 1980 Jun 15;140(1):129-42 PMID: 6251230
  8. Expression of early genes of origin-defective mutants of simian virus 40.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3898-902 PMID: 6254000
  9. Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6491-5 PMID: 6256739
  10. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  11. SV40-transformed simian cells support the replication of early SV40 mutants.
    Cell. 1981 Jan;23(1):175-82 PMID: 6260373
  12. Simian virus 40 tandem repeated sequences as an element of the early promoter.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):943-7 PMID: 6262784
  13. Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):100-4 PMID: 6264425
  14. Simian virus 40 early mRNA's contain multiple 5' termini upstream and downstream from a Hogness-Goldberg sequence; a shift in 5' termini during the lytic cycle is mediated by large T antigen.
    J Virol. 1981 Oct;40(1):224-40 PMID: 6270376
  15. The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants.
    Nucleic Acids Res. 1981 Nov 25;9(22):6047-68 PMID: 6273820
  16. A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication.
    Nucleic Acids Res. 1981 Dec 11;9(23):6231-50 PMID: 6275353
  17. Territorial limits and functional anatomy of the simian virus 40 replication origin.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):381-5 PMID: 6281769
  18. An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase.
    Nucleic Acids Res. 1982 Apr 10;10(7):2309-21 PMID: 6283476
  19. Initiation of SV40 DNA replication in vivo: location and structure of 5' ends of DNA synthesized in the ori region.
    Cell. 1982 Apr;28(4):767-79 PMID: 6178514
  20. Regulatory mutants of simian virus 40. Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes.
    J Mol Biol. 1982 Apr 15;156(3):531-48 PMID: 6288959
  21. Deletion mapping of DNA regions required for SV40 early region promoter function in vivo.
    J Mol Appl Genet. 1982;1(5):457-81 PMID: 6296253
  22. Non-contiguous segments of the polyoma genome required in cis for DNA replication.
    J Mol Biol. 1982 Nov 15;161(4):533-50 PMID: 6296399
  23. DNA primase activity associated with DNA polymerase alpha from Xenopus laevis ovaries.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7209-13 PMID: 6961403
  24. Definition of the simian virus 40 early promoter region and demonstration of a host range bias in the enhancement effect of the simian virus 40 72-base-pair repeat.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):721-5 PMID: 6298771
  25. Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2221-5 PMID: 6403945
  26. Transcription in vivo from SV40 early promoter deletion mutants without repression by large T antigen.
    J Mol Appl Genet. 1983;2(1):127-35 PMID: 6842119
  27. Tight association of DNA primase with a subspecies of mouse DNA polymerase alpha.
    J Biol Chem. 1983 Jun 10;258(11):6698-700 PMID: 6406487
  28. The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter.
    Nucleic Acids Res. 1983 Apr 25;11(8):2447-64 PMID: 6304653
  29. Enhancer elements.
    Cell. 1983 Jun;33(2):313-4 PMID: 6305503
  30. A DNA primase from mouse cells. Purification and partial characterization.
    J Biol Chem. 1983 Aug 25;258(16):9845-9 PMID: 6885774
  31. Polyomavirus origin for DNA replication comprises multiple genetic elements.
    J Virol. 1983 Sep;47(3):586-99 PMID: 6312083
  32. Mutational dissection of the 21 bp repeat region of the SV40 early promoter reveals that it contains overlapping elements of the early-early and late-early promoters.
    Nucleic Acids Res. 1984 Jan 25;12(2):915-32 PMID: 6320125
  33. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.
    J Natl Cancer Inst. 1968 Aug;41(2):351-7 PMID: 4299537
  34. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
    J Virol. 1972 Oct;10(4):591-8 PMID: 4343541
  35. Cell killing by simian virus 40: impairment of membrane formation and function.
    J Virol. 1977 Mar;21(3):872-9 PMID: 191651
  36. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  37. The genome of simian virus 40.
    Science. 1978 May 5;200(4341):494-502 PMID: 205947
  38. Construction of a viable SV40 variant containing two functional origins of DNA replication.
    Cell. 1978 Apr;13(4):791-8 PMID: 207442
  39. Mapping of the late promoter of simian virus 40.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):23-7 PMID: 6320166
  40. Simian virus 40 late promoter region able to initiate simian virus 40 early gene transcription in the absence of the simian virus 40 origin sequence.
    J Virol. 1984 Apr;50(1):163-73 PMID: 6321788
  41. An SV40 "enhancer trap" incorporates exogenous enhancers or generates enhancers from its own sequences.
    Cell. 1984 Apr;36(4):983-92 PMID: 6323032
  42. An enhancer sequence from bovine papilloma virus DNA consists of two essential regions.
    Nucleic Acids Res. 1984 Mar 26;12(6):2901-16 PMID: 6324133
  43. Critical spatial requirement within the origin of simian virus 40 DNA replication.
    J Virol. 1984 Jul;51(1):91-6 PMID: 6328047
  44. Short and long range activation by the SV40 enhancer.
    Nucleic Acids Res. 1984 Jul 25;12(14):5589-608 PMID: 6087293
  45. DNA replication and chromatin structure of simian virus 40 insertion mutants.
    Mol Cell Biol. 1984 Aug;4(8):1499-507 PMID: 6092914
  46. The simian virus 40 minimal origin and the 72-base-pair repeat are required simultaneously for efficient induction of late gene expression with large tumor antigen.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6335-9 PMID: 6093097
  47. A viable simian virus 40 variant that carries a newly generated sequence reiteration in place of the normal duplicated enhancer element.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6652-6 PMID: 6093109
  48. Polyoma virus DNA replication requires an enhancer.
    Nature. 1984 Nov 15-21;312(5991):242-6 PMID: 6095089
  49. Mutational analysis of simian virus 40 large T antigen DNA binding sites.
    EMBO J. 1984 Dec 20;3(13):3247-55 PMID: 6098470
  50. Polyomavirus enhancer contains multiple redundant sequence elements that activate both DNA replication and gene expression.
    Mol Cell Biol. 1985 Apr;5(4):649-58 PMID: 2985964
  51. trans Activation of the simian virus 40 late transcription unit by T-antigen.
    Mol Cell Biol. 1985 Jun;5(6):1391-9 PMID: 2993862
  52. Sequences involved in determining the locations of the 5' ends of the late RNAs of simian virus 40.
    J Virol. 1985 Dec;56(3):1002-13 PMID: 2999418
  53. Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences.
    J Virol. 1986 Jan;57(1):138-44 PMID: 3001340
  54. A putative origin of replication of plasmids derived from Epstein-Barr virus is composed of two cis-acting components.
    Mol Cell Biol. 1985 Aug;5(8):1822-32 PMID: 3018528
  55. Analysis of an activatable promoter: sequences in the simian virus 40 late promoter required for T-antigen-mediated trans activation.
    Mol Cell Biol. 1985 Aug;5(8):1859-69 PMID: 3018532
  56. Heterogeneity and 5'-terminal structures of the late RNAs of simian virus 40.
    J Mol Biol. 1978 Dec 25;126(4):813-46 PMID: 218021
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.10.3513-3522.1986
Published
1986-10-00
Pages
3513-22
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367100
Subset
IM
Grants
NCI NIH HHS · CA-07175 · United States
NCI NIH HHS · CA-22443 · United States
NCI NIH HHS · CA-37208 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.10.3513-3522.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2