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PMID: 6324133 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An enhancer sequence from bovine papilloma virus DNA consists of two essential regions.

Nucleic acids research ·Vol. 12 ·No. 6 ·1984-03-26 ·Pages 2901-16

Weiher H, Botchan MR

Abstract

A comprehensive structural analysis of an enhancer sequence from bovine papilloma virus DNA is presented based on the construction and functional analysis of 20 mutant derivatives. The results, obtained in CV-1 tissue culture cells, show that this enhancer is a small genetic element--only 40 bp in length--that contains two essential regions. The two regions exhibit homology to each other and to DNA fragments from other viral genomes that also act as enhancers in the assay used. However, there is a certain latitude in the sequences that have enhancer activity in CV-1 cells, even within the critical regions. The results are discussed with respect to the model that enhancers are binding sites for tissue specific transcription factors. The formation of Z-DNA might be involved in the enhancement process. However, single base pair transitions in an 8 base pair stretch of alternating purines and pyrimidines within the BPV enhancer which conserve this pattern destroy enhancer function.

MeSH Terms
Animals Base Sequence Bovine papillomavirus 1/genetics Cattle Cell Line Chlorocebus aethiops Cloning, Molecular DNA Restriction Enzymes DNA, Viral/genetics Escherichia coli/genetics Lung Mutation Papillomaviridae/genetics Plasmids
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weiher H
Botchan M R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.6.2901
Published
1984-03-26
Pages
2901-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318714
Subset
IM
Grants
NCI NIH HHS · CA 30490 · United States
Databases
GENBANK
X00473
External Links
PubMed source DOI: 10.1093/nar/12.6.2901 PMC Full Text View journal details
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