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PMID: 3520552 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a telomere-binding activity from yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 11 ·1986-06-00 ·Pages 3713-7

Berman J, Tachibana CY, Tye BK

Abstract

In yeast, the ends of the chromosomes (telomeres) terminate in repeated poly(C1-3A) sequences. We have identified a yeast activity that binds specifically to these poly(C1-3A) repeats. An agarose gel binding assay was used to detect and characterize this activity in cell extracts using both cloned telomere DNA and yeast genomic DNA as substrates. The activity appears to bind specifically to poly(C1-3A) sequences, despite their different primary sequences, yet does not bind specifically to telomeric repeats, such as poly(C4A2), poly(C4A4), and poly (C1-8T) from other lower eukaryotes.

MeSH Terms
Base Sequence Chromosomes/metabolism,ultrastructure Cloning, Molecular DNA, Fungal/metabolism DNA-Binding Proteins/metabolism Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Species Specificity
Chemicals
DNA, Fungal DNA-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berman J
Tachibana C Y
Tye B K
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.11.3713
Published
1986-06-00
Pages
3713-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323593
Subset
IM
Grants
NIGMS NIH HHS · GM34190 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.11.3713 PMC Full Text View journal details
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