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PMID: 3012521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transient replication of bovine papilloma virus type 1 plasmids: cis and trans requirements.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 11 ·1986-06-00 ·Pages 3609-13

Lusky M, Botchan MR

Abstract

A transient assay has been used to study bovine papilloma virus type 1 (BPV-1) replication. We show that BPV-1 early replication occurs faster than cellular DNA synthesis. Initial replication events are dependent on a gene product(s), encoded by the BPV-1 E1 open reading frame. Mutational analysis of the viral upstream regulatory region shows the requirement of two domains in cis for replication. Domain one, located outside of the viral 69% transforming fragment, is an enhancer-like activity and can be replaced by other known viral enhancers. Domain two lies within sequences previously defined as plasmid maintenance sequence 1. The apparent requirement for a proximal enhancer function for replication may explain why certain BPV-1 constructions, when linked to bacterial plasmid sequences, can be maintained extrachromosomally while others cannot.

MeSH Terms
Bovine papillomavirus 1/genetics,growth & development Chromosome Mapping DNA, Superhelical/biosynthesis DNA, Viral/biosynthesis Enhancer Elements, Genetic Gene Expression Regulation Papillomaviridae/growth & development Plasmids Replicon Time Factors Transcription, Genetic Virus Replication
Chemicals
DNA, Superhelical DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lusky M
Botchan M R
References (31)
31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.11.3609
Published
1986-06-00
Pages
3609-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323572
Subset
IM
Grants
NCI NIH HHS · CA 30490 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.11.3609 PMC Full Text View journal details
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