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PMID: 6330534 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

New procedure for DNA transfection with polycation and dimethyl sulfoxide.

Molecular and cellular biology ·Vol. 4 ·No. 6 ·1984-06-00 ·Pages 1172-4

Kawai S, Nishizawa M

Abstract

A new procedure for DNA transfection has been developed in a system of chicken embryo fibroblast cells and cloned Rous sarcoma virus DNA by using a polycation reagent as a mediator to adsorb DNA to the cell surface and dimethyl sulfoxide as an agent to facilitate the uptake of adsorbed DNA by the cells. In this new, simple, and convenient polycation-dimethyl sulfoxide transfection, which requires no carrier DNA even with small amounts of DNA, the number of transformed cell foci induced by Rous sarcoma virus DNA was proportional to the dose of the transfecting DNA, and chicken embryo fibroblast cells were successfully transformed by v-src-containing subgenomic DNA as well.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Cells, Cultured Chick Embryo Cloning, Molecular DNA, Viral/genetics Dimethyl Sulfoxide/pharmacology Fibroblasts/physiology Hexadimethrine Bromide/pharmacology Kinetics Polyamines/pharmacology Transfection/drug effects
Chemicals
DNA, Viral Polyamines Hexadimethrine Bromide Dimethyl Sulfoxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kawai S
Nishizawa M
References (21)
21 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.6.1172-1174.1984
Published
1984-06-00
Pages
1172-4
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368888
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.4.6.1172-1174.1984 PMC Full Text View journal details
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