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PMID: 2183026 Published · ppublish English Journal Article

Yeast alpha 2 repressor positions nucleosomes in TRP1/ARS1 chromatin.

Molecular and cellular biology ·Vol. 10 ·No. 5 ·1990-05-00 ·Pages 2247-60

Roth SY, Dean A, Simpson RT

Abstract

The yeast alpha 2 repressor suppresses expression of a-mating-type-specific genes in haploid alpha and diploid a/alpha cell types. We inserted the alpha 2-binding site into the multicopy TRP1/ARS1 yeast plasmid and examined the effects of alpha 2 on the chromatin structure of the derivative plasmids in alpha cells, and a/alpha cells. Whereas no effect on nucleosome position was observed in a cells, nucleosomes were precisely and stably positioned over sequences flanking the alpha 2 operator in alpha and a/alpha cells. In addition, when the alpha 2 operator was located upstream of the TRP1 gene, an extended array of positioned nucleosomes was formed in alpha cells and a/alpha cells, with formation of a nucleosome not present in a cells, and TRP1 mRNA production was substantially reduced. These data indicate that alpha 2 causes a positioning of nucleosomes over sequences proximal to its operator in TRP1/ARS1 chromatin and suggest that changes in chromatin structure may be related to alpha 2 repression of cell-type-specific genes.

MeSH Terms
Gene Expression Regulation, Fungal Mating Factor Micrococcal Nuclease/pharmacology Nucleosomes/ultrastructure Operator Regions, Genetic Peptides/physiology Plasmids Regulatory Sequences, Nucleic Acid Repressor Proteins/physiology Saccharomyces cerevisiae/genetics,physiology Transcription Factors/physiology Tryptophan
Chemicals
Nucleosomes Peptides Repressor Proteins Transcription Factors Mating Factor Tryptophan Micrococcal Nuclease
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roth S Y
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Dean A
Simpson R T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.5.2247-2260.1990
Published
1990-05-00
Pages
2247-60
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360572
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.5.2247-2260.1990 PMC Full Text View journal details
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