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PMID: 2233725 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The Saccharomyces cerevisiae SIN3 gene, a negative regulator of HO, contains four paired amphipathic helix motifs.

Molecular and cellular biology ·Vol. 10 ·No. 11 ·1990-11-00 ·Pages 5927-36

Wang H, Clark I, Nicholson PR, Herskowitz I, Stillman DJ

Abstract

The SIN3 gene (also known as SDI1) is a negative regulator of the yeast HO gene. Mutations in SIN3 suppress the requirement for the SWI5 activator for expression of the yeast HO gene and change the normal asymmetric pattern of HO expression in mother and daughter cells. Furthermore, the in vitro DNA-binding activity of several DNA-binding proteins is reduced in extracts prepared from sin3 mutants. We have cloned the SIN3 gene and determined that a haploid strain with a SIN3 gene disruption is viable. We determined the sequence of the SIN3 gene, which is predicted to encode a 175-kDa polypeptide with four paired amphipathic helix motifs. These motifs have been identified in the myc family of helix-loop-helix DNA-binding proteins and in the TPR family of regulatory proteins. The SIN3 transcript was mapped, and it was determined that the SIN3 transcript was absent in stationary-phase cells. Immunofluorescence microscopy with anti-SIN3 antibody demonstrated that SIN3 protein was present in nuclei. A comparison of restriction map and sequence data revealed that SIN3 is the same as regulatory genes UME4 and RPD1.

Related Genes
HO SIN3
MeSH Terms
Amino Acid Sequence Base Sequence DNA-Binding Proteins/genetics Fungal Proteins/genetics Genes, Fungal Genes, Regulator Genetic Complementation Test Molecular Sequence Data Mutation Protein Conformation RNA, Messenger/genetics Restriction Mapping Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid
Chemicals
DNA-Binding Proteins Fungal Proteins RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wang H
Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132.
Clark I
Nicholson P R
Herskowitz I
Stillman D J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.11.5927-5936.1990
Published
1990-11-00
Pages
5927-36
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361389
Subset
IM
Grants
NIAID NIH HHS · AI18738 · United States
NIGMS NIH HHS · GM39067 · United States
NCRR NIH HHS · RR01865-05 · United States
Databases
GENBANK
M36822
External Links
PubMed source DOI: 10.1128/mcb.10.11.5927-5936.1990 PMC Full Text View journal details
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