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PMID: 2263626 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vitro regulation of a SIN3-dependent DNA-binding activity by stimulatory and inhibitory factors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 24 ·1990-12-00 ·Pages 9761-5

Wang H, Stillman DJ

Abstract

The yeast SIN3 gene (also known as SDII, is a known negative regulator of the yeast HO gene. A DNA-binding activity, called SDP1, which binds to the HO promoter, is absent in extracts prepared from sin3 mutants and has been proposed to function as a repressor. We show that SIN3 does not encode SDP1 and that SDP1 DNA-binding activity is modulated in vitro by two factors, an inhibitory factor, I-SDP1, and a stimulatory factor, S-SDP1. I-SDP1 acts as an in vitro inhibitor of the SDP1 DNA-binding activity. Restoration of the DNA-binding activity is achieved by inclusion of a stimulatory factor, S-SDP1, which copurifies with the SIN3 protein. SDP1 DNA-binding activity was restored by treating a protein fraction containing SDP1 and I-SDP1 with the dissociating agent formamide.

Related Genes
HO SIN3
MeSH Terms
Base Sequence DNA-Binding Proteins/genetics Formamides/pharmacology Genes, Fungal Genes, Regulator Kinetics Molecular Sequence Data Oligonucleotide Probes Promoter Regions, Genetic Saccharomyces cerevisiae/genetics,metabolism
Chemicals
DNA-Binding Proteins Formamides Oligonucleotide Probes formamide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang H
Department of Cellular, Viral, and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132.
Stillman D J
References (30)
30 references, click to expand
  1. Activation of the yeast HO gene by release from multiple negative controls.
    Cell. 1987 Feb 27;48(4):567-77 PMID: 3545494
  2. Molecular genetics of yeast mating type.
    Annu Rev Genet. 1982;16:439-500 PMID: 6760802
  3. Cell cycle regulation of SW15 is required for mother-cell-specific HO transcription in yeast.
    Cell. 1987 May 22;49(4):549-58 PMID: 3552251
  4. TUF, the yeast DNA-binding factor specific for UASrpg upstream activating sequences: identification of the protein and its DNA-binding domain.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3648-52 PMID: 3295867
  5. Transcriptional regulation in the yeast life cycle.
    Science. 1987 Sep 4;237(4819):1162-70 PMID: 3306917
  6. Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Jan;8(1):210-25 PMID: 3275867
  7. Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer.
    Proc Natl Acad Sci U S A. 1988 Apr;85(7):2120-4 PMID: 3281162
  8. Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.
    Cell. 1988 Apr 22;53(2):211-7 PMID: 3129195
  9. Characterization of a transcription factor involved in mother cell specific transcription of the yeast HO gene.
    EMBO J. 1988 Feb;7(2):485-94 PMID: 3284746
  10. A movable and regulable inactivation function within the steroid binding domain of the glucocorticoid receptor.
    Cell. 1988 Sep 23;54(7):1073-80 PMID: 2843290
  11. I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
    Science. 1988 Oct 28;242(4878):540-6 PMID: 3140380
  12. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
    Cell. 1989 Mar 10;56(5):777-83 PMID: 2493990
  13. Life cycle of the budding yeast Saccharomyces cerevisiae.
    Microbiol Rev. 1988 Dec;52(4):536-53 PMID: 3070323
  14. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence.
    Cell. 1989 Aug 11;58(3):537-44 PMID: 2503252
  15. A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes.
    Genes Dev. 1989 Jul;3(7):921-35 PMID: 2673922
  16. The yeast transcription activator PRTF, a homolog of the mammalian serum response factor, is encoded by the MCM1 gene.
    Genes Dev. 1989 Jul;3(7):936-45 PMID: 2550323
  17. Yeast repressor alpha 2 binds to its operator cooperatively with yeast protein Mcm1.
    Mol Cell Biol. 1989 Nov;9(11):5228-30 PMID: 2689875
  18. Identification of negative regulatory genes that govern the expression of early meiotic genes in yeast.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):10018-22 PMID: 2690066
  19. A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis.
    Cell. 1990 Jan 26;60(2):307-17 PMID: 2404612
  20. Snap helix with knob and hole: essential repeats in S. pombe nuclear protein nuc2+.
    Cell. 1990 Jan 26;60(2):319-28 PMID: 2297790
  21. Transcriptional regulation by dimerization: two sides to an incestuous relationship.
    Cell. 1990 Apr 6;61(1):9-11 PMID: 2180585
  22. Identification of a Saccharomyces cerevisiae DNA-binding protein involved in transcriptional regulation.
    Mol Cell Biol. 1990 Apr;10(4):1743-53 PMID: 2181283
  23. Mutations that disrupt DNA binding and dimer formation in the E47 helix-loop-helix protein map to distinct domains.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4722-6 PMID: 2112746
  24. Direct selection for mutants with increased K+ transport in Saccharomyces cerevisiae.
    Genetics. 1990 Jun;125(2):313-20 PMID: 2199313
  25. REB1, a yeast DNA-binding protein with many targets, is essential for growth and bears some resemblance to the oncogene myb.
    Mol Cell Biol. 1990 Oct;10(10):5226-34 PMID: 2204808
  26. The Saccharomyces cerevisiae SIN3 gene, a negative regulator of HO, contains four paired amphipathic helix motifs.
    Mol Cell Biol. 1990 Nov;10(11):5927-36 PMID: 2233725
  27. The determination of mother cell-specific mating type switching in yeast by a specific regulator of HO transcription.
    EMBO J. 1987 Jan;6(1):243-8 PMID: 15981333
  28. Identification of silencer binding proteins from yeast: possible roles in SIR control and DNA replication.
    EMBO J. 1987 Feb;6(2):461-7 PMID: 15981337
  29. Asymmetry and directionality in production of new cell types during clonal growth: the switching pattern of homothallic yeast.
    Cell. 1979 Jun;17(2):371-81 PMID: 378408
  30. Both positive and negative regulators of HO transcription are required for mother-cell-specific mating-type switching in yeast.
    Cell. 1987 Feb 27;48(4):579-87 PMID: 3028642
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.24.9761
Published
1990-12-00
Pages
9761-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55253
Subset
IM
Grants
NIGMS NIH HHS · GM39067 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.24.9761 PMC Full Text View journal details
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